TOBFAC: The database of tobacco transcription factors
Family: AP2
Minimum number of tobacco AP2 genes: 35
Count of tobacco AP2 sequences: 60
Pfam accession: AP2
SHOULD possess AP2 domain and COULD possess B3 SNF2_N domains
The APETELA2(AP2) family is part of the larger AP2/ERF superfamily that also contains the ERF and RAV families. The superfamily is defined by the presence of the approximately 60 amino acid AP2 domain that is involved in DNA binding and may be related to site-specific endonucleases that are found in bacteria and bacteriophages . In general, the ERF proteins have one AP2 domain, the AP2 proteins have two and the RAV proteins contain an AP2 domain together with another DNA binding domain, the B3 domain .
For our purposes, the tobacco AP2 genes contain two AP2 domains and lack the conserved WLG and the AEIRD parts of ERFs. Instead they have a conserved YLG.
Okamuro, JK; Caster, B; Villarroel, R; Van Montagu, M; Jofuku, KD. The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 1997. 94(13):7076-81 PMID: 9192694
Nole-Wilson S, Krizek BA. DNA binding properties of the Arabidopsis floral development protein AINTEGUMENTA. Nucleic Acids Res. 2000 Nov 1;28(21):4076-82. Erratum in: Nucleic Acids Res 2001 Mar 1;29(5):1261. PMID: 11058102
Shigyo, M; Hasebe, M; Ito, M. Molecular evolution of the AP2 subfamily. Gene 2006. 366(2):256-65 PMID: 16388920
Shigyo, M; Ito, M. Analysis of gymnosperm two-AP2-domain-containing genes. Dev. Genes Evol. 2004. 214(3):105-14 PMID: 14986134
Weigel, D. The APETALA2 domain is related to a novel type of DNA binding domain. Plant Cell 1995. 7(4):388-9 PMID: 7773013
R1 repeat contains WESHI or YEAH at the 5 ‘ end and LAALKY or YDRAA at the 3 ‘ end.
R2 repeat contains WEAR or WQAR at the 5 ‘ end and YDIAAI or NAVT at the 3 ‘ end.
Number of contigs: 53
Number of singlets: 12
R1 + R2 – 5
R1 – 8
R1 5 prime – 10
R1 3 prime – 5
R2 5 prime – 6
R2 3 prime – 10
R2 – 4
R1 + R2 5 prime – 12
Total minimum number – 35
| Family | Genbank ID | Name |
| AP2 | AY461432 | NtANTL (AP2 family) |
Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko