AS2 Genomic sequences

Minimum number of tobacco AS2 genes: 75

Count of tobacco AS2 sequences: 91

Pfam accession: PF03195 DUF260

The LOB domain contains conserved blocks of amino acids that identify the LBD gene family. In particular, a conserved C-x(2)-C-x(6)-C-x(3)-C motif, which is defining feature of the LOB domain, is present in all LBD proteins. It is possible that this motif forms a new zinc finger.

The lateral organ boundaries (LOB) gene is expressed at the adaxial base of initiating lateral organs and encodes a plant-specific protein of unknown function. The N-terminal one half of the LOB protein contains a conserved approximately 100-amino acid domain (the LOB domain) that is present in 42 other Arabidopsis thaliana proteins and in proteins from a variety of other plant species. Genes encoding LOB domain (LBD) proteins are expressed in a variety of temporal- and tissue-specific patterns, suggesting that they may function in diverse processes.

The ASYMMETRIC LEAVES2 (AS2) gene of Arabidopsis thaliana is involved in the establishment of the leaf venation system, which includes the prominent midvein, as well as in the development of a symmetric lamina. The gene product also represses the expression of class 1 knox homeobox genes in leaves. We have characterized the AS2 gene, which appears to encode a novel protein with cysteine repeats (designated the C-motif) and a leucine-zipper-like sequence in the amino-terminal half of the primary sequence. The Arabidopsis genome contains 42 putative genes that potentially encode proteins with conserved amino acid sequences that include the C-motif and the leucine-zipper-like sequence in the amino-terminal half. Thus, the AS2 protein belongs to a novel family of proteins that we have designated the AS2 family. Members of this family except AS2 also have been designated ASLs (AS2-like proteins). Transcripts of AS2 were detected mainly in adaxial domains of cotyledonary primordia. Green fluorescent protein-fused AS2 was concentrated in plant cell nuclei. Overexpression of AS2 cDNA in transgenic Arabidopsis plants resulted in upwardly curled leaves, which differed markedly from the downwardly curled leaves generated by loss-of-function mutation of AS2. Our results suggest that AS2 functions in the transcription of a certain gene(s) in plant nuclei and thereby controls the formation of a symmetric flat leaf lamina and the establishment of a prominent midvein and other patterns of venation.

Papers

Iwakawa H, Ueno Y, Semiarti E, Onouchi H, Kojima S, Tsukaya H, Hasebe M, Soma T, Ikezaki M, Machida C, Machida Y. The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for formation of a symmetric flat leaf lamina, encodes a member of a novel family of proteins characterized by cysteine repeats and a leucine zipper. Plant Cell Physiol. 2002 May;43(5):467-78. PMID: 12040093



Number of contigs: 85

Number of singlets: 8

Number of N terminal – 16

Number of C terminal – 21

Number of full – 54

Total minimum number – 75




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
AS2_1 [comment=N terminal] ET041720
AS2_2 [comment=C terminal] ET045114
AS2_3 [comment=C terminal] ET048522
AS2_4 [comment=full] ET043985
ET046481
AS2_5 [comment=C terminal] ET042613
ET044435
AS2_6 [comment=full] ET042514
ET044450
AS2_7 [comment=full] ET044315
ET045105
AS2_8 [comment=full] ET042841
ET043339
AS2_9 [comment=full] ET045132
ET050177
AS2_10 [comment=full] ET046109
ET045054
AS2_11 [comment=C terminal] ET047730
ET049568
AS2_12 [comment=N terminal] ET048019
ET051612
AS2_13 [comment=C terminal] ET045896
ET051289
AS2_14 [comment=full] ET049043
ET049795
AS2_15 [comment=C terminal] ET046212
ET046211
AS2_16 [comment=full] ET049615
ET048526
AS2_17 [comment=C terminal] ET048040
ET047970
AS2_18 [comment=N terminal] ET051241
ET044857
AS2_19 [comment=full] ET041885
ET041884
AS2_20 [comment=C terminal] ET050252
ET050253
AS2_21 [comment=N terminal] ET042342
ET044631
AS2_22 [comment=N terminal] ET051886
ET044443
AS2_23 [comment=N terminal] ET042723
ET042724
AS2_24 [comment=N terminal] ET044173
ET050489
ET044172
AS2_25 [comment=full] ET045245
ET043950
ET043793
AS2_26 [comment=full] ET047466
ET043304
ET043321
AS2_27 [comment=C terminal] ET041699
ET046826
ET041698
AS2_28 [comment=full] ET049028
ET048258
ET045216
AS2_29 [comment=full] ET049843
ET049899
ET043898
AS2_30 [comment=C terminal] ET047183
ET047937
ET046369
AS2_31 [comment=full] ET042693
ET042786
ET046517
AS2_32 [comment=C terminal] ET049315
ET051582
ET042300
AS2_33 [comment=C terminal] ET049432
ET042958
ET042598
AS2_34 [comment=full] ET049184
ET042143
ET043118
AS2_35 [comment=full] ET047386
ET045311
ET049722
AS2_36 [comment=C terminal] ET049402
ET047483
ET048063
AS2_37 [comment=full] ET045032
ET049914
ET045031
AS2_38 [comment=full] ET045714
ET050968
ET047469
AS2_39 [comment=N terminal] ET051506
ET051504
ET043907
ET049316
AS2_40 [comment=full] ET042387
ET050883
ET049948
ET043857
AS2_41 [comment=full] ET046076
ET044057
ET045126
ET045930
AS2_42 [comment=full] ET050526
ET046732
ET043352
ET045098
AS2_43 [comment=N terminal] ET045871
ET044420
ET051581
ET050376
AS2_44 [comment=C terminal] ET045377
ET046605
ET046498
ET046354
AS2_45 [comment=full] ET042402
ET050892
ET042401
ET044493
AS2_46 [comment=full] ET046463
ET047558
ET047547
ET051147
AS2_47 [comment=full] ET046897
ET044280
ET042504
ET045113
AS2_48 [comment=N terminal] ET046461
ET049692
ET050730
ET050728
AS2_49 [comment=C terminal] ET043885
ET051767
ET045047
ET049256
AS2_50 [comment=full] ET049523
ET046238
ET049519
ET043409
AS2_51 [comment=full] ET046465
ET043924
ET044025
ET049949
AS2_52 [comment=C terminal] ET044392
ET049109
ET050517
ET050516
AS2_53 [comment=C terminal] ET042112
ET044588
ET045854
ET044195
AS2_54 [comment=full] ET044163
ET046017
ET044164
ET051769
AS2_55 [comment=full] ET050156
ET051847
ET046439
ET048694
AS2_56 [comment=full] ET047512
ET048458
ET048022
ET048345
ET047513
AS2_57 [comment=full] ET049926
ET042029
ET045094
ET044650
ET045093
AS2_58 [comment=full] ET049880
ET043908
ET041792
ET041791
ET044571
AS2_59 [comment=full] ET048306
ET048483
ET044424
ET049965
ET051199
AS2_60 [comment=full] ET042680
ET050186
ET046643
ET047021
ET042679
AS2_61 [comment=full] ET048424
ET045689
ET045812
ET045050
ET051274
ET050630
AS2_62 [comment=N terminal] ET045048
ET041679
ET048773
ET047252
ET051516
ET042472
AS2_63 [comment=full] ET050367
ET044971
ET051406
ET051411
ET045372
ET045623
AS2_64 [comment=full] ET051811
ET042513
ET044352
ET045867
ET045227
ET043201
AS2_65 [comment=full] ET042243
ET042793
ET042244
ET046275
ET050799
ET051408
ET051414
AS2_66 [comment=full] ET049253
ET045947
ET042321
ET042323
ET045946
ET049052
ET048359
AS2_67 [comment=full] ET051723
ET043720
ET050318
ET043036
ET050194
ET041713
ET045230
AS2_68 [comment=full] ET049831
ET046250
ET041842
ET044922
ET051505
ET049832
ET050849
AS2_69 [comment=C terminal] ET049317
ET051006
ET046649
ET050693
ET049558
ET043969
ET044734
ET044616
AS2_70 [comment=full] ET048294
ET048419
ET050243
ET046104
ET050713
ET050714
ET046283
ET048867
AS2_71 [comment=N terminal] ET045936
ET045652
ET045655
ET050742
ET045188
ET045949
ET049115
ET045634
AS2_72 [comment=N terminal] ET050835
ET051823
ET050887
ET042065
ET049572
ET045750
ET051822
ET046315
AS2_73 [comment=full] ET050321
ET043040
ET048162
ET051050
ET049116
ET043039
ET050124
ET050268
AS2_74 [comment=full] ET046802
ET049789
ET046801
ET049148
ET044141
ET044147
ET051561
ET051563
ET049788
AS2_75 [comment=full] ET045229
ET045811
ET049036
ET049377
ET049376
ET044218
ET044203
ET050439
ET044219
AS2_76 [comment=N terminal] ET047552
ET045175
ET044852
ET046064
ET046063
ET043713
ET044508
ET047553
ET046062
AS2_77 [comment=full] ET051112
ET048114
ET045680
ET045990
ET044061
ET046070
ET046071
ET049976
ET043235
ET048115
AS2_78 [comment=full] ET044398
ET050920
ET047961
ET044427
ET048780
ET050014
ET047960
ET047026
ET044428
ET043688
ET048781
AS2_79 [comment=full] ET045236
ET044001
ET043883
ET047242
ET047819
ET051713
ET047818
ET047358
ET045396
ET046057
ET050706
AS2_80 [comment=full] ET047200
ET049185
ET047280
ET042128
ET042230
ET047522
ET049492
ET048899
ET046653
ET051614
ET046652
ET047279
AS2_81 [comment=full] ET043988
ET051413
ET047221
ET048938
ET042279
ET050413
ET051084
ET044162
ET043149
ET043987
ET042692
ET046007
AS2_82 [comment=full] ET044183
ET050106
ET045360
ET042906
ET047954
ET043341
ET047732
ET051856
ET047733
ET050105
ET047955
ET045361
ET043342
AS2_83 [comment=full] ET046911
ET049760
ET045717
ET050145
ET051161
ET048098
ET049759
ET044621
ET051462
ET048623
ET042993
ET051160
ET045716
ET048075
AS2_84 [comment=full] ET048037
ET050415
ET046862
ET045180
ET046796
ET047892
ET050227
ET046296
ET046636
ET044520
ET042605
ET043591
ET043968
ET046635
ET050537
ET046125
AS2_85 [comment=C terminal] ET043455
AS2_86 [comment=full] ET043178
AS2_87 [comment=full] ET050176
AS2_88 [comment=C terminal] ET045586
AS2_89 [comment=C terminal] ET047864
AS2_90 [comment=N terminal] ET047362
AS2_91 [comment=N terminal] ET042599


Tobacco published genes related to transcription factor family AS2
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu