AUX-IAA Genomic sequences

Minimum number of tobacco AUX-IAA genes: 35

Count of tobacco AUX-IAA sequences: 52

Pfam accession: AUX_IAA

SHOULD possess AUX_IAA domain but SHOULD NOT possess Auxin_resp domain

The Aux/IAA family of genes are key regulators of auxin-modified gene expression. The plant hormone auxin (indole-3-acetic acid, IAA) regulates diverse cellular and developmental responses in plants, including cell division, expansion, differentiation and patterning of embryo responses. Auxin can regulate the gene expression of several families, including GH3 and SAUR, as well as Aux/IAA itself. The Aux/IAA proteins act as repressors of auxin-induced gene expression, possibly through modulating the activity of DNA-binding auxin response factors (ARFs). Aux/IAA and ARF are thought to interact through C-terminal protein-protein interaction domains found in both Aux/IAA and ARF.

Recent evidence suggests that Aux/IAA proteins can also mediate light responses. Some members of the AUX/IAA family are longer and contain an N-terminal DNA binding domain and may have an early function in the establishment of vascular and body patterns in embryonic and post-embryonic development in some plants.

Aux/IAA proteins are short-lived nuclear proteins that repress expression of primary/early auxin response genes in protoplast transfection assays. Repression is thought to result from Aux/IAA proteins dimerizing with auxin response factor (ARF) transcriptional activators that reside on auxin-responsive promoter elements, referred to as AuxREs. Most Aux/IAA proteins contain four conserved domains, designated domains I, II, III, and IV. Domain II and domains III and IV play roles in protein stability and dimerization, respectively. A clear function for domain I had not been established. Results reported here indicate that domain I in Aux/IAA proteins is an active repression domain that is transferable and dominant over activation domains. An LxLxL motif within domain I is important for conferring repression. The dominance of Aux/IAA repression domains over activation domains in ARF transcriptional activators provides a plausible explanation for the repression of auxin response genes via ARF-Aux/IAA dimerization on auxin-responsive promoters.

Papers

Liscum, E; Reed, JW. Genetics of Aux/IAA and ARF action in plant growth and development. Plant Mol. Biol. 2002. 49(3-4):387-400 PMID: 12036262

Tiwari, SB; Hagen, G; Guilfoyle, TJ. Aux/IAA proteins contain a potent transcriptional repression domain. Plant Cell 2004. 16(2):533-43 PMID: 14742873

Tiwari, SB; Wang, XJ; Hagen, G; Guilfoyle, TJ. AUX/IAA proteins are active repressors, and their stability and activity are modulated by auxin. Plant Cell 2001.13(12):2809-22 PMID: 11752389

Reed JW. Roles and activities of Aux/IAA proteins in Arabidopsis. Trends Plant Sci. 2001 Sep;6(9):420-5. PMID: 11544131



Number of contigs: 36

Number of singlets: 16

Number of N terminal – 17

Number of C terminal – 25

Number of full – 10

Total minimum number – 35




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
AUX-IAA_1 [comment=N terminal] ET051512
AUX-IAA_2 [comment=N terminal] ET050966
AUX-IAA_3 [comment=C terminal] ET047925
ET043417
AUX-IAA_4 [comment=N terminal] ET044713
ET045177
AUX-IAA_5 [comment=N terminal] ET046719
ET046718
AUX-IAA_6 [comment=C terminal] ET045682
ET047546
AUX-IAA_7 [comment=full] ET049994
ET049993
AUX-IAA_8 [comment=N terminal] ET050930
ET050928
AUX-IAA_9 [comment=C terminal] ET044997
ET047903
AUX-IAA_10 [comment=C terminal] ET048233
ET045712
AUX-IAA_11 [comment=C terminal] ET051686
ET044196
AUX-IAA_12 [comment=C terminal] ET042695
ET042694
AUX-IAA_13 [comment=N terminal] ET050408
ET051375
AUX-IAA_14 [comment=N terminal] ET046673
ET046672
AUX-IAA_15 [comment=C terminal] ET047782
ET044099
ET047781
AUX-IAA_16 [comment=C terminal] ET045244
ET044490
ET045243
AUX-IAA_17 [comment=full] ET044494
ET050360
ET047282
AUX-IAA_18 [comment=C terminal] ET049098
ET044027
ET051511
AUX-IAA_19 [comment=N terminal] ET044044
ET041684
ET050211
AUX-IAA_20 [comment=full] ET042883
ET042884
ET047388
AUX-IAA_21 [comment=C terminal] ET049073
ET048879
ET043246
AUX-IAA_22 [comment=C terminal] ET047898
ET043104
ET047897
AUX-IAA_23 [comment=N terminal] ET047754
ET048897
ET041773
ET047753
AUX-IAA_24 [comment=N terminal] ET048009
ET043387
ET051116
ET044544
AUX-IAA_25 [comment=C terminal] ET051855
ET050608
ET041685
ET051471
AUX-IAA_26 [comment=C terminal] ET046777
ET047387
ET043381
ET051352
AUX-IAA_27 [comment=N terminal] ET046173
ET049009
ET042780
ET044777
AUX-IAA_28 [comment=full] ET051257
ET051258
ET046248
ET051549
AUX-IAA_29 [comment=N terminal] ET045151
ET045150
ET043372
ET043647
AUX-IAA_30 [comment=full] ET049596
ET049134
ET049595
ET049133
AUX-IAA_31 [comment=full] ET050929
ET051478
ET051901
ET051881
AUX-IAA_32 [comment=C terminal] ET047091
ET048398
ET046151
ET046150
ET047597
AUX-IAA_33 [comment=full] ET051726
ET049860
ET043876
ET051725
ET049859
AUX-IAA_34 [comment=full] ET047363
ET048369
ET043889
ET050311
ET046788
AUX-IAA_35 [comment=C terminal] ET048200
ET043517
ET051716
ET051850
ET048199
ET051657
ET051849
AUX-IAA_36 [comment=C terminal] ET042080
ET043621
ET044501
ET044221
ET044205
ET044146
ET044502
ET044222
AUX-IAA_37 [comment=C terminal] ET048137
AUX-IAA_38 [comment=full] ET042340
AUX-IAA_39 [comment=C terminal] ET049078
AUX-IAA_40 [comment=full] ET044127
AUX-IAA_41 [comment=C terminal] ET048839
AUX-IAA_42 [comment=C terminal] ET050725
AUX-IAA_43 [comment=N terminal] ET051525
AUX-IAA_44 [comment=C terminal] ET048400
AUX-IAA_45 [comment=N terminal] ET044210
AUX-IAA_46 [comment=N terminal] ET042339
AUX-IAA_47 [comment=C terminal] ET049776
AUX-IAA_48 [comment=C terminal] ET048716
AUX-IAA_49 [comment=C terminal] ET044495
AUX-IAA_50 [comment=N terminal] ET047078
AUX-IAA_51 [comment=C terminal] ET049064
AUX-IAA_52 [comment=N terminal] ET042645


Tobacco published genes related to transcription factor family AUX-IAA
Family Genbank ID Name
AUX-IAA AF123505 Nt-iaa2.5 deduced protein
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu