CAMTA Genomic sequences

Minimum number of tobacco CAMTA genes: 6

Count of tobacco CAMTA sequences: 9

Pfam accession: CG-1 and IQ domains

SHOULD possess CG-1 IQ domains and COULD   possess Ank domain

CG-1 domains are highly conserved domains of about 130 amino-acid residues containing a predicted bipartite NLS and named after a partial cDNA clone isolated from parsley encoding a sequence-specific DNA-binding protein. CG-1 domains are associated with CAMTA proteins (for CAlModulin -binding Transcription Activator) that are transcription factors containing a calmodulin -binding domain and ankyrins (ANK) motifs.

Calmodulin (CaM) is recognized as a major calcium sensor and orchestrator of regulatory events through its interaction with a diverse group of cellular proteins. Three classes of recognition motifs exist for many of the known CaM binding proteins; the IQ motif as a consensus for Ca2+-independent binding and two related motifs for Ca2+-dependent binding, termed 18-14 and 1-5-10 based on the position of conserved hydrophobic residues.

Screening of cDNA expression libraries derived from plants exposed to stress, with 35S-labeled recombinant calmodulin as a probe, revealed a new family of proteins containing a transcription activation domain and two types of DNA-binding domains designated the CG-1 domain and the transcription factor immunoglobulin domain, ankyrin repeats, and a varying number of IQ calmodulin-binding motifs. Based on domain organization and amino acid sequence comparisons, similar proteins, with the same domain organization, were identified in the genomes of other multicellular organisms including human, Drosophila, and Caenorhabditis, whereas none were found in the complete genomes of single cell eukaryotes and prokaryotes. This family of proteins was designated calmodulin-binding transcription activators (CAMTAs).

Papers

Bouché, N; Scharlat, A; Snedden, W; Bouchez, D; Fromm, H. A novel family of calmodulin-binding transcription activators in multicellular organisms. J. Biol. Chem. 2002. 277(24):21851-61 PMID: 11925432

Reddy, AS; Reddy, VS; Golovkin, M. A calmodulin binding protein from Arabidopsis is induced by ethylene and contains a DNA-binding motif. Biochem. Biophys. Res. Commun. 2000. 279(3):762-9 PMID: 11162426

da Costa e Silva, O. CG-1, a parsley light-induced DNA-binding protein. PLANT MOLECULAR BIOLOGY 25:921-924 1994 PMID: 8075408



Number of contigs: 6

Number of singlets: 5

Number of N terminal – 3

Number of C terminal – 6

Total minimum number – 6




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
CAMTA_1 [comment=N terminal] ET043867
ET044411
CAMTA_2 [comment=C terminal] ET045545
ET045554
CAMTA_3 [comment=N terminal] ET049960
ET047792
ET049959
CAMTA_4 [comment=C terminal] ET049716
ET047644
ET048309
ET049216
CAMTA_5 [comment=C terminal] ET044440
ET049341
ET049349
ET044338
ET043216
CAMTA_6 [comment=C terminal] ET043224
CAMTA_7 [comment=C terminal] ET042137
CAMTA_8 [comment=C terminal] ET042488
CAMTA_9 [comment=N terminal] ET043504


Tobacco published genes related to transcription factor family CAMTA
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu