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TOBFAC: The database of tobacco transcription factors

Family: CAMTA

Minimum number of tobacco CAMTA genes: 6

Count of tobacco CAMTA sequences: 9

Pfam accession: CG-1 and IQ domains

SHOULD possess CG-1 IQ domains and COULD   possess Ank domain

CG-1 domains are highly conserved domains of about 130 amino-acid residues containing a predicted bipartite NLS and named after a partial cDNA clone isolated from parsley encoding a sequence-specific DNA-binding protein. CG-1 domains are associated with CAMTA proteins (for CAlModulin -binding Transcription Activator) that are transcription factors containing a calmodulin -binding domain and ankyrins (ANK) motifs.

Calmodulin (CaM) is recognized as a major calcium sensor and orchestrator of regulatory events through its interaction with a diverse group of cellular proteins. Three classes of recognition motifs exist for many of the known CaM binding proteins; the IQ motif as a consensus for Ca²⁺-independent binding and two related motifs for Ca²⁺-dependent binding, termed 18-14 and 1-5-10 based on the position of conserved hydrophobic residues.

Screening of cDNA expression libraries derived from plants exposed to stress, with 35S-labeled recombinant calmodulin as a probe, revealed a new family of proteins containing a transcription activation domain and two types of DNA-binding domains designated the CG-1 domain and the transcription factor immunoglobulin domain, ankyrin repeats, and a varying number of IQ calmodulin-binding motifs. Based on domain organization and amino acid sequence comparisons, similar proteins, with the same domain organization, were identified in the genomes of other multicellular organisms including human, Drosophila, and Caenorhabditis, whereas none were found in the complete genomes of single cell eukaryotes and prokaryotes. This family of proteins was designated calmodulin-binding transcription activators (CAMTAs).

Papers

Bouché, N; Scharlat, A; Snedden, W; Bouchez, D; Fromm, H. A novel family of calmodulin-binding transcription activators in multicellular organisms. J. Biol. Chem. 2002. 277(24):21851-61 PMID: 11925432

Reddy, AS; Reddy, VS; Golovkin, M. A calmodulin binding protein from Arabidopsis is induced by ethylene and contains a DNA-binding motif. Biochem. Biophys. Res. Commun. 2000. 279(3):762-9 PMID: 11162426

da Costa e Silva, O. CG-1, a parsley light-induced DNA-binding protein. PLANT MOLECULAR BIOLOGY 25:921-924 1994 PMID: 8075408

Number of contigs: 6

Number of singlets: 5

Number of N terminal – 3

Number of C terminal – 6

Total minimum number – 6

Search sequences and info

Transcription factor sequences

Tobacco published genes related to transcription factor family CAMTA

Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu