Dof Genomic sequences Dof Phylogeny file Dof Phylogeny file with bootstrap Dof Phylogeny JPEG

Minimum number of tobacco C2C2-Dof genes: 46

Count of tobacco C2C2-Dof sequences: 48

Pfam accession: zf-Dof

SHOULD possess zf-Dof domain

Dof (DNA-binding with one finger) domain proteins are plant-specific transcription factors with a highly conserved DNA-binding domain, which presumably includes a single C(2)-C(2) zinc finger. During the past decade, numerous Dof domain proteins have been identified in both monocots and dicots including maize, barley, wheat, rice, tobacco, Arabidopsis, pumpkin, potato, and pea. Biochemical, molecular biological and molecular genetic analyses revealed that Dof domain proteins function as a transcriptional activator or a repressor involved in diverse plant-specific biological processes. Although more physiological roles of Dof domain proteins would be elucidated in future because of numerous Dof domain proteins in plants, it is already evident that the Dof domain proteins play critical roles as transcriptional regulators in plant growth and development.

Papers

Yanagisawa S Dof domain proteins: Plant-specific transcription factors associated with diverse phenomena unique to plants PLANT AND CELL PHYSIOLOGY 45 (4): 386-391 2004 PMID: 15111712

Yanagisawa S, Akiyama A, Kisaka H, Uchimiya H, Miwa T Metabolic engineering with Dof1 transcription factor in plants: Improved nitrogen assimilation and growth under low-nitrogen conditions PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 101 (20): 7833-7838 2004 PMID: 15136740

Umemura Y, Ishiduka T, Yamamoto R, Esaka M The Dof domain, a zinc finger DNA-binding domain conserved only in higher plants, truly functions as a Cys2/Cys2 Zn finger domain PLANT JOURNAL 37 (5): 741-749 2004 PMID: 14871313

Baumann,K., De Paolis,A., Costantino,P. and Gualberti,G. The DNA binding site of the Dof protein NtBBF1 is essential for tissue-specific and auxin-regulated expression of the rolB oncogene in plants PLANT CELL 11 (3): 323-334 1999 PMID: 10072394

De Paolis,A., Sabatini,S., De Pascalis,L., Costantino,P. and Capone,I. A rolB regulatory factor belongs to a new class of single zinc finger plant proteins PLANT JOURNAL 10 (2): 215-223 1996 PMID: 8771779

Lijavetzky, D; Carbonero, P; Vicente-Carbajosa, J. Genome-wide comparative phylogenetic analysis of the rice and Arabidopsis Dof gene families. BMC Evol. Biol. 2003. 3:17 PMID: 12877745



Number of N terminal - 3

Number of C terminal - 3

Number of full – 43

Total minimum number – 46




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
Dof_1 [comment=full] ET045676
ET048318
Dof_2 [comment=full except last 5 amino acids] ET045909
ET049874
Dof_3 [comment=full identical to BBF1.1] ET050254
ET041951
Dof_4 [comment=5 prime to CNNY] ET044171
ET047385
Dof_5 [comment=missing last 10 amino acids] ET046307
ET047541
Dof_6 [comment=full] ET043936
ET047940
Dof_7 [comment=full] ET051717
ET043608
Dof_8 [comment=second half] ET045321
ET049450
ET049111
Dof_9 [comment=full] ET043465
ET044992
ET051771
Dof_10 [comment=full] ET042820
ET051567
ET049814
Dof_11 [comment=full] ET045228
ET048779
ET046968
Dof_12 [comment=full identical to N.tabacum zinc finger protein, BBF3.] ET047393
ET048031
ET043083
Dof_13 [comment=full] ET048176
ET051238
ET043106
Dof_14 [comment=full] ET049904
ET049522
ET043434
Dof_15 [comment=full] ET042623
ET044393
ET044648
Dof_16 [comment=full] ET046126
ET044084
ET050170
ET049521
Dof_17 [comment=full] ET046488
ET046834
ET050704
ET046842
Dof_18 [comment=full ] ET048173
ET046073
ET043383
ET043487
Dof_19 [comment=full] ET049138
ET044397
ET046749
ET044311
Dof_20 [comment=full] ET050478
ET051345
ET051346
ET043951
Dof_21 [comment=full] ET047511
ET045787
ET047693
ET045604
Dof_22 [comment=full] ET049413
ET050336
ET049285
ET045645
Dof_23 [comment=full] ET049786
ET043559
ET050825
ET045200
Dof_24 [comment=full] ET048941
ET042226
ET050649
ET041962
Dof_25 [comment=full] ET048539
ET047536
ET044721
ET045644
Dof_26 [comment=full] ET050066
ET047672
ET046888
ET045649
ET047134
Dof_27 [comment=full] ET043703
ET049225
ET051903
ET050893
ET048587
Dof_28 [comment=full] ET045908
ET043002
ET042091
ET042924
ET043673
Dof_29 [comment=full] ET051367
ET050975
ET051328
ET044707
ET051840
Dof_30 [comment=full] ET046415
ET051634
ET047101
ET050354
ET050299
Dof_31 [comment=full] ET043366
ET045876
ET045503
ET043396
ET043367
Dof_32 [comment=full] ET047942
ET044246
ET049938
ET042586
ET044247
Dof_33 [comment=full] ET045863
ET043811
ET051281
ET049411
ET045864
ET047405
Dof_34 [comment=full] ET050016
ET043402
ET049065
ET044130
ET047711
ET047710
ET043403
Dof_35 [comment=full] ET045548
ET045556
ET049124
ET048238
ET046609
ET042410
ET049674
Dof_36 [comment=full] ET048055
ET047620
ET044100
ET044101
ET046322
ET047890
ET045520
ET050109
Dof_37 [comment=full] ET046583
ET044889
ET044887
ET046374
ET044045
ET044888
ET046384
ET041903
Dof_38 [comment=full] ET051743
ET046706
ET051780
ET050641
ET043606
ET043360
ET051173
ET051744
Dof_39 [comment=full] ET049489
ET051252
ET044694
ET050303
ET051253
ET044693
ET046477
ET046342
Dof_40 [comment=full] ET044109
ET046730
ET046729
ET046068
ET046069
ET047130
ET047423
ET049652
Dof_41 [comment=full] ET047438
ET049158
ET047437
ET049958
ET049143
ET051091
ET049157
ET047607
ET048730
Dof_42 [comment=full] ET051003
ET047491
ET041881
ET043686
ET049161
ET043313
ET044325
ET049608
ET043881
Dof_43 [comment=full] ET043980
ET049026
ET049588
ET051407
ET051412
ET051087
ET042295
ET051088
ET049331
ET048778
ET045732
Dof_44 [comment=5 prime half] ET044316
Dof_45 [comment=3 prime half] ET047250
Dof_46 [comment=full] ET049495
Dof_47 [comment=3 prime three quarters] ET050818
Dof_48 [comment=5 prime end] ET043122


Tobacco published genes related to transcription factor family Dof
Family Genbank ID Name
Dof AJ009594 BBF1
Dof X97947 BBF3
Dof X97946 NtBBF2b
Dof X97945 NtBBF2a
Dof X97942 NtBBF1.1
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu