EIL Genomic sequences

Minimum number of tobacco EIL genes: 6

Count of tobacco EIL sequences: 7

Pfam accession: EIN3

SHOULD possess EIN3 domain

ETHYLENE INSENSITIVE3 (EIN3) are transcription factors involved in the ethylene signal transduction pathway in Arabidopsis and higher plants. Two full-length cDNA clones, pVR-EIL1 and pVR-EIL2, encoding EIN3-LIKE proteins were isolated by reverse transcriptase-polymerase chain reaction and by screening the cDNA library of mung bean (Vigna radiata) hypocotyls. VR-EIL1 and VR-EIL2 share 70% identity and display varying degrees of sequence conservation (39%¨C65%) with previously isolated EIN3 homologs from Arabidopsis, tobacco (Nicotiana tabacum) and tomato (Lycopersicon esculentum) plants. Gel retardation assay revealed that both VR-EILs were able to interact specifically with optimal binding sequence-1, the recently identified optimal binding sequence for tobacco TEIL, with the binding of VR-EIL2 being more efficient than that of VR-EIL1. Transient expression analysis using a VR-EIL::smGFP fusion gene in onion (Allium cepa) epidermal cells indicated that the VR-EIL proteins were effectively targeted to the nucleus. The fusion protein of VR-EIL2 with GAL4 DNA-binding domain strongly activated transcription of a reporter gene in yeast cells, and an essential domain for transcription-stimulating activity was localized to the amino-terminal acidic region that consists of 50 amino acid residues. In contrast with what has been previously found in EIN3- and TEIL-overexpressing Arabidopsis plants, transgenic tobacco seedlings expressing the VR-EIL genes under the control of cauliflower mosaic virus 35S promoter did not exhibit a constitutive triple response.

Papers

Lee JH, Kim WT. Molecular and biochemical characterization of VR-EILs encoding mung bean ETHYLENE INSENSITIVE3-LIKE proteins. Plant Physiol. 2003 Jul;132(3):1475-88. PMID: 12857828

Solano, R; Stepanova, A; Chao, Q; Ecker, JR. Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1. Genes Dev. 1998.12(23):3703-14 PMID: 9851977

Yamasaki, K; Kigawa, T; Inoue, M; Yamasaki, T; Yabuki, T; Aoki, M; Seki, E; Matsuda, T; Tomo, Y; Terada, T; Shirouzu, M; Tanaka, A; Seki, M; Shinozaki, K; Yokoyama, S. Solution structure of the major DNA-binding domain of Arabidopsis thaliana ethylene-insensitive3-like3. J. Mol. Biol. 2005. 348(2):253-64 PMID: 15811366

Chao, Q; Rothenberg, M; Solano, R; Roman, G; Terzaghi, W; Ecker, JR. Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins. Cell 1997. 89(7):1133-44 PMID: 9215635



Number of contigs: 6

Number of singlets: 0

Number of N terminal – 6

Number of C terminal – 1

Number of full – 0

Total minimum number – 6




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
EIL_1 [comment=similar to NtEIL3 and NtEIL4 5 prime end to NPVGP] ET042848
EIL_2 [comment=similar to EIL5 5 prime end till FDRNGPA] ET041696
EIL_3 [comment=5 prime half of gene. Rather divergent] ET041756
ET051008
ET041755
ET051007
EIL_4 [comment=5 prime end] ET042252
ET047116
ET041880
ET046716
ET046717
ET044350
EIL_5 [comment=5 prime end and most of the protein] ET044144
ET048278
EIL_6 [comment=5 prime end and most of gene very similar to NtEIL3] ET043925
ET048437
ET049516
ET045827
ET049454
EIL_7 [comment=3 prime end very similar to NtEIL5] ET050092


Tobacco published genes related to transcription factor family EIL
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu