ERF Genomic sequences ERF Phylogeny file ERF Phylogeny file with bootstrap ERF Phylogeny JPEG ERF Phylogeny PDF

Minimum number of tobacco ERF genes: 239

Count of tobacco ERF sequences: 241

Pfam accession: AP2

SHOULD possess AP2 domain and COULD   possess B3 SNF2_N domains

The ERF family of transcription factors was first discovered in tobacco and is a large multigene family that plays important roles in regulating a variety of plant processes . These include responses to both biotic and abiotic stresses as well as processes during growth and development. The ERF family is part of the larger AP2/ERF superfamily that also contains the AP2 and RAV families. The superfamily is defined by the presence of the approximately 60 amino acid AP2 domain that is involved in DNA binding and may be related to site-specific endonucleases that are found in bacteria and bacteriophages . In general, the ERF proteins have one AP2 domain, the AP2 proteins have two and the RAV proteins contain an AP2 domain together with another DNA binding domain, the B3 domain . Tobacco has a long history of ERF-related research, as the first four recorded genes (EREBP1-4) were isolated from tobacco . These genes are implicated in regulating responses to pathogens and ethylene. Other ERF genes from tobacco appear to play roles in jasmonate signaling (NtORC1 and NtJAP1) , pathogen attack (ERF5) and osmotic stress (TSI1) and members of the DREB subfamily such as DREB1A probably play roles in dehydration and cold responses.

Papers

Okamuro, JK; Caster, B; Villarroel, R; Van Montagu, M; Jofuku, KD. The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 1997. 94(13):7076-81 PMID: 9192694

Magnani, E; Sjölander, K; Hake, S. From endonucleases to transcription factors: evolution of the AP2 DNA binding domain in plants. Plant Cell 2004. 16(9):2265-77 PMID: 15319480

Ohme-Takagi, M; Shinshi, H. Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. Plant Cell 1995. 7(2):173-82 PMID: 7756828

Riechmann, JL; Meyerowitz, EM. The AP2/EREBP family of plant transcription factors. Biol. Chem. 1998. 379(6):633-46 PMID: 9687012

Weigel, D. The APETALA2 domain is related to a novel type of DNA binding domain. Plant Cell 1995. 7(4):388-9 PMID: 7773013



Number of contigs: 232

Number of singlets: 9

Number of N terminal - 10

Number of C terminal - 7

Number of full – 224

Total minimum number – 239

Of the 241 predicted genes, ten are fragmentary and contain just the N-terminal part of the ERF domain and seven the C-terminal portion. Based on their amino acid sequences and phylogenetic positions, a maximum of two N-terminal fragments could potentially correspond to C-terminal fragments and the minimum number of ERF genes in tobacco is therefore 239.




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
ERF_1 ET046444
ERF_2 ET048054
ERF_3 ET049326
ERF_4 ET042507
ERF_5 ET046034
ERF_6 ET048973
ERF_7 ET046148
ERF_8 ET050960
ERF_9 ET047674
ERF_10 [alias=JAP1] ET047304
ERF_11 ET046932
ERF_12 ET046646
ERF_13 ET049578
ERF_14 ET048593
ERF_15 ET042716
ERF_16 ET045455
ERF_17 ET043515
ERF_18 ET047980
ET051623
ERF_19 ET050420
ET044380
ERF_20 ET046366
ET050409
ERF_21 ET045038
ET045037
ERF_22 ET046916
ET044745
ERF_23 ET047488
ET050664
ERF_24 ET045470
ET049862
ERF_25 ET041816
ET041815
ERF_26 ET051437
ET049785
ERF_27 ET048668
ET050316
ERF_28 ET049424
ET046490
ERF_29 ET043856
ET043432
ERF_30 ET049628
ET047697
ERF_31 ET047254
ET047763
ERF_32 [alias=EREBP2] ET044111
ET046963
ERF_33 ET051655
ET049408
ERF_34 ET046020
ET047305
ERF_35 ET043428
ET044916
ERF_36 ET049095
ET045241
ERF_37 ET046124
ET045694
ERF_38 ET047356
ET047355
ERF_39 ET050857
ET050856
ERF_40 ET051659
ET051658
ERF_41 ET048127
ET050645
ERF_42 ET051537
ET047652
ERF_43 ET043616
ET043070
ERF_44 ET044883
ET047498
ERF_45 ET044082
ET044715
ERF_46 ET043763
ET046383
ERF_47 ET047229
ET045642
ERF_48 ET045181
ET048618
ERF_49 ET042860
ET043724
ERF_50 ET042362
ET046982
ERF_51 ET048310
ET048872
ERF_52 ET051048
ET047727
ERF_53 ET045673
ET044239
ERF_54 ET048480
ET048580
ERF_55 ET046628
ET046627
ERF_56 ET049012
ET051492
ERF_57 ET051219
ET042991
ERF_58 ET043678
ET047251
ERF_59 ET043765
ET048649
ERF_60 ET042194
ET042704
ERF_61 ET047163
ET045415
ERF_62 ET048534
ET046484
ERF_63 ET051768
ET049017
ERF_64 ET042972
ET048954
ERF_65 ET046984
ET042727
ERF_66 ET042968
ET043679
ERF_67 ET045112
ET045440
ERF_68 ET046744
ET050117
ERF_69 ET048877
ET048573
ERF_70 ET048409
ET047179
ET044114
ERF_71 ET043384
ET043333
ET042326
ERF_72 ET048974
ET050320
ET046129
ERF_73 ET048279
ET043121
ET045950
ERF_74 ET050869
ET050236
ET044291
ERF_75 ET048809
ET042757
ET050302
ERF_76 ET048259
ET044917
ET046317
ERF_77 ET049659
ET047826
ET044499
ERF_78 ET050547
ET047455
ET049048
ERF_79 ET046512
ET043322
ET051845
ERF_80 ET049107
ET049947
ET050800
ERF_81 ET045760
ET044321
ET047224
ERF_82 ET044961
ET042447
ET049059
ERF_83 ET045425
ET042259
ET049773
ERF_84 ET051787
ET045342
ET051438
ERF_85 ET049515
ET051738
ET046326
ERF_86 ET045997
ET042904
ET045905
ERF_87 ET051553
ET049121
ET046226
ERF_88 ET046684
ET045881
ET046683
ERF_89 ET050554
ET047794
ET049962
ERF_90 ET050100
ET048936
ET050004
ERF_91 ET049582
ET049015
ET041977
ERF_92 [comment=Contains three ERF domains] ET049396
ET042584
ET049397
ERF_93 ET051282
ET047951
ET044695
ERF_94 ET051746
ET051361
ET044789
ERF_95 [alias=ACRE1] ET043930
ET043520
ET049763
ERF_96 ET049093
ET045359
ET048141
ERF_97 ET041735
ET051320
ET042076
ERF_98 [alias=ERF47] ET043454
ET046344
ERF_99 ET047934
ET043622
ET047933
ERF_100 ET051251
ET051819
ET050834
ERF_101 ET051701
ET050720
ET043753
ERF_102 ET049491
ET050019
ET048708
ERF_103 ET049929
ET049928
ET051124
ERF_104 ET051447
ET046115
ET047471
ERF_105 ET049273
ET050579
ET044627
ERF_106 ET044389
ET043785
ET046084
ERF_107 ET047422
ET051247
ET042305
ERF_108 ET042651
ET043097
ET047571
ERF_109 ET048174
ET051667
ET042121
ERF_110 ET045840
ET045365
ET045366
ERF_111 ET046228
ET043627
ET047291
ERF_112 ET044576
ET042307
ET044575
ERF_113 ET047319
ET051800
ET047678
ERF_114 [alias=ACRE111a] ET041771
ET041770
ET041726
ERF_115 ET041702
ET041703
ET046143
ET045430
ERF_116 ET049173
ET045838
ET045837
ET050617
ERF_117 ET045391
ET045133
ET047859
ET045715
ERF_118 ET046005
ET046142
ET046072
ET045248
ERF_119 ET045270
ET047293
ET045999
ET045271
ERF_120 ET051389
ET043603
ET051387
ET043604
ERF_121 [alias=ERF5] ET048690
ET047532
ET048848
ERF_122 ET044845
ET043380
ET047964
ET044846
ERF_123 [alias=EREBP1] ET042119
ET045731
ET045643
ERF_124 ET045422
ET051400
ET049004
ET051151
ERF_125 [alias=ERF1] ET047762
ET043533
ET045781
ERF_126 ET049548
ET044223
ET044206
ET042413
ERF_127 ET045982
ET042213
ET044304
ET045980
ERF_128 ET047636
ET047635
ET044787
ET044533
ERF_129 ET046347
ET047392
ET048412
ET050903
ERF_130 ET050759
ET049599
ET049918
ET050582
ERF_131 ET047994
ET045839
ET048532
ET045790
ERF_132 ET050648
ET050157
ET048373
ET047879
ERF_133 ET044086
ET048500
ET047215
ET047214
ERF_134 ET046294
ET044258
ET042595
ET043909
ERF_135 ET045751
ET041828
ET047525
ET041827
ERF_136 ET045835
ET050342
ET044612
ET045834
ERF_137 ET049741
ET045070
ET047126
ET045071
ERF_138 ET046223
ET047808
ET046075
ET046074
ERF_139 ET042931
ET042930
ET044317
ET043830
ERF_140 ET049497
ET050072
ET044348
ET051296
ERF_141 ET047418
ET045860
ET041984
ET042943
ERF_142 ET045075
ET048916
ET046161
ET044679
ERF_143 [alias=Tsi1] ET043165
ET045380
ET043314
ERF_144 ET047373
ET043427
ET049195
ET048524
ERF_145 ET046540
ET046884
ET044029
ET050897
ERF_146 ET045796
ET051139
ET046133
ET051140
ERF_147 ET046433
ET048863
ET046432
ET044727
ERF_148 ET046271
ET049060
ET046270
ET047804
ERF_149 ET044253
ET049094
ET042522
ET046106
ERF_150 ET051677
ET050583
ET051486
ET043280
ERF_151 ET045678
ET044387
ET051687
ET046766
ET051423
ERF_152 ET050097
ET050680
ET049829
ET050656
ET044604
ERF_153 ET044168
ET044365
ET044469
ET047440
ET050827
ERF_154 ET046089
ET046753
ET043837
ET048993
ET043955
ERF_155 ET047990
ET050907
ET044129
ET046079
ET050908
ERF_156 ET049624
ET049638
ET043776
ET042878
ET045411
ERF_157 ET045195
ET051732
ET046500
ET049736
ET050351
ERF_158 ET050612
ET044190
ET048215
ET045629
ET049392
ERF_159 ET049425
ET045349
ET049985
ET043952
ET049066
ERF_160 ET050228
ET049334
ET042944
ET047688
ET047687
ERF_161 ET046087
ET045948
ET047331
ET051595
ET046086
ERF_162 ET048397
ET046592
ET048637
ET048636
ET048042
ERF_163 ET046043
ET047420
ET050703
ET048168
ET041929
ERF_164 ET043878
ET048081
ET044578
ET043457
ET048696
ERF_165 ET050057
ET048758
ET049235
ET049198
ET050084
ERF_166 ET047241
ET045215
ET041946
ET041947
ET049734
ERF_167 ET050191
ET042739
ET048667
ET046305
ET046532
ERF_168 ET043852
ET048201
ET043802
ET043152
ET051148
ERF_169 ET044186
ET044579
ET047378
ET044580
ET050980
ERF_170 ET041748
ET041747
ET047884
ET046623
ET044237
ERF_171 ET041860
ET045561
ET050875
ET047506
ET050876
ERF_172 ET051268
ET042746
ET042741
ET042742
ET051269
ERF_173 ET043000
ET048887
ET048401
ET046232
ET042999
ERF_174 ET045414
ET046509
ET048621
ET048276
ET047479
ERF_175 ET044088
ET051131
ET047927
ET047823
ET044089
ET045465
ERF_176 ET046333
ET045814
ET047580
ET045343
ET051306
ET051601
ERF_177 ET051169
ET050577
ET042068
ET041776
ET044700
ET044699
ERF_178 ET045213
ET047592
ET045129
ET048504
ET046051
ET050255
ERF_179 ET045581
ET045974
ET047451
ET051373
ET042408
ET049264
ERF_180 ET051259
ET043704
ET041998
ET041715
ET041999
ET041714
ERF_181 ET046194
ET043361
ET047868
ET043375
ET046193
ET045527
ERF_182 ET044880
ET049841
ET045022
ET050708
ET051187
ET042696
ERF_183 ET050946
ET041829
ET041825
ET041826
ET051183
ET045889
ERF_184 ET042753
ET047752
ET047526
ET048937
ET050122
ET047527
ERF_185 ET046887
ET043804
ET046398
ET041961
ET045110
ET041960
ERF_186 ET044873
ET044328
ET047195
ET049293
ET042947
ET042948
ERF_187 ET044753
ET046848
ET047559
ET042005
ET046847
ET044754
ERF_188 ET043944
ET045776
ET042223
ET046101
ET047452
ET048232
ERF_189 ET043310
ET044336
ET050306
ET050307
ET046098
ET050984
ERF_190 ET042220
ET044743
ET048264
ET042221
ET047608
ET044742
ERF_191 ET045826
ET051559
ET051556
ET050393
ET051810
ET051774
ERF_192 ET048679
ET048894
ET042628
ET046616
ET042626
ET046617
ERF_193 [alias=EREBP5] ET051229
ET043818
ET041718
ET041719
ET051228
ERF_194 ET050651
ET045688
ET045784
ET045935
ET049429
ET047061
ERF_195 ET044053
ET051715
ET047936
ET044214
ET045843
ET043364
ERF_196 [alias=S25XP1] ET041744
ET050333
ET041736
ET044678
ET041737
ERF_197 ET042212
ET050756
ET050136
ET048686
ET045636
ET042211
ERF_198 ET045855
ET047623
ET049890
ET050925
ET045856
ET048467
ET047622
ERF_199 ET045805
ET046048
ET051366
ET043549
ET045817
ET049382
ET047909
ERF_200 ET047729
ET051315
ET045063
ET047943
ET044960
ET051267
ET047728
ERF_201 ET047065
ET042497
ET042775
ET042495
ET047064
ET051890
ET042496
ERF_202 [alias=ACRE111b] ET047777
ET051448
ET050002
ET048678
ET050873
ET045397
ERF_203 ET047992
ET048845
ET042417
ET048346
ET042416
ET047991
ET048707
ERF_204 ET041716
ET044102
ET041717
ET050335
ET047389
ET047426
ERF_205 ET043489
ET051496
ET043513
ET046634
ET045721
ET043488
ET047888
ERF_206 ET051874
ET046131
ET049237
ET051175
ET051174
ET051184
ET051185
ERF_207 ET047977
ET041758
ET048074
ET043746
ET047232
ET041757
ET044003
ERF_208 ET041730
ET050403
ET045912
ET048135
ET051616
ET045756
ET041731
ET044779
ERF_209 ET048342
ET045637
ET047138
ET047139
ET045618
ET051518
ET044708
ET045617
ERF_210 ET049552
ET048186
ET044949
ET044050
ET051730
ET042196
ET044049
ET049296
ERF_211 ET047495
ET045458
ET043827
ET047931
ET045154
ET046473
ET050288
ET047932
ERF_212 ET049163
ET044864
ET044176
ET045310
ET045223
ET047000
ET049262
ET045943
ERF_213 ET051015
ET051014
ET046010
ET043211
ET041722
ET044038
ET048165
ET041765
ERF_214 ET042448
ET046324
ET050705
ET050593
ET043236
ET051493
ET048184
ET046323
ERF_215 [alias=CEF1] ET048039
ET050446
ET048144
ET042762
ET045145
ET046080
ET043094
ERF_216 [alias=EREBP4] ET050161
ET042084
ET044405
ET046338
ET050169
ET051249
ET042743
ERF_217 ET044079
ET045077
ET049856
ET044080
ET048076
ET047261
ET049217
ET045078
ERF_218 ET049654
ET049774
ET044803
ET046570
ET042419
ET042421
ET043847
ET045891
ET044289
ERF_219 ET050993
ET044543
ET045771
ET044542
ET045874
ET050961
ET050848
ET045869
ET046574
ERF_220 ET051735
ET048670
ET046994
ET042006
ET051692
ET043237
ET048477
ET051674
ET045963
ERF_221 [alias=ORC1] ET045797
ET047322
ET047594
ET047595
ET050581
ET041898
ET050606
ET043963
ERF_222 ET043069
ET043882
ET043228
ET043068
ET050983
ET050979
ET045190
ET046925
ET050326
ERF_223 ET050752
ET047070
ET050753
ET045431
ET048312
ET045432
ET048701
ET048313
ET045446
ERF_224 ET045651
ET051105
ET050048
ET043485
ET050435
ET046343
ET044447
ET047516
ET048158
ERF_225 ET046410
ET042990
ET048116
ET043730
ET046065
ET046521
ET046411
ET046066
ET046067
ET046387
ERF_226 [alias=EREBP3] ET049746
ET045131
ET045647
ET044582
ET047539
ET043354
ET050068
ET049969
ET044482
ERF_227 ET045109
ET051022
ET047820
ET045663
ET045184
ET051004
ET047458
ET044985
ET049453
ERF_228 ET050129
ET042045
ET041919
ET050472
ET046154
ET046153
ET044213
ET051596
ET045172
ET046935
ERF_229 [alias=EREBP6] ET043047
ET047125
ET047738
ET047739
ET047904
ET043046
ET045859
ET047838
ET051037
ERF_230 ET051194
ET051192
ET051193
ET046093
ET046910
ET050938
ET046146
ET046147
ET046003
ET046004
ERF_231 ET041766
ET042011
ET050977
ET049447
ET050240
ET041767
ET044055
ET042814
ET042627
ET041723
ET048546
ET042010
ERF_232 ET042755
ET043841
ET041848
ET051294
ET041849
ET048654
ET043293
ET049110
ET042756
ET050560
ET041850
ET048655
ERF_233 [comment=contains three ERF domains] ET047047
ET049664
ET048128
ET046836
ET043651
ET046835
ET050615
ET047105
ET047048
ET048129
ET049665
ET043652
ET047104
ERF_234 ET045543
ET041743
ET042297
ET046919
ET045552
ET041742
ET044656
ET046105
ET050309
ET050308
ET042296
ET041838
ET047939
ET043636
ET050060
ET047399
ET042306
ET045882
ET043805
ET050609
ERF_235 ET044701
ERF_236 ET044724
ERF_237 ET049506
ERF_238 ET046892
ERF_239 ET051776
ERF_240 ET043758


Tobacco published genes related to transcription factor family ERF
Family Genbank ID Name
ERF AY655738 ERF5
ERF AY286010 CEF1
ERF DQ403814 DREB1A
ERF AJ299252 ap2/ERF47
ERF AY627866 EREBP-6
ERF AY627865 EREBP-5
ERF AF211531 ACRE111B
ERF AF211530 ACRE111A
ERF AF211527 ACRE1
ERF AX700837 Patent WO03012096
ERF TOBBY4D EREBP-2
ERF TOBBY4C EREBP-4
ERF TOBBY4B EREBP-3
ERF AF058827 TSI1
ERF AB024575 EREBP-5
ERF AF057373 EREBP1-2 allele
ERF TOBBY4A ERF1
ERF NTU81157 S25-XP1
ERF CQ808982 ORC1
ERF CQ808845 JAP1
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu