GARP-G2 Genomic sequences

Minimum number of tobacco GARP-G2-like genes: 64

Count of tobacco GARP-G2-like sequences: 84

Pfam accession: Myb_DNA-binding

The GLK proteins are members of the recently categorized GARP superfamily of transcription factors (Riechmann et al., 2000) defined by G2 in maize; the Arabidopsis RESPONSE REGULATOR-B (ARR-B) proteins (Imamura et al., 1999); and the PHOSPHATE STARVATION RESPONSE1 (PSR1) protein of Chlamydomonas (Wykoff et al., 1999). In the case of G2, three of the four defining features of most transcription factors have been verified experimentally in heterologous systems. G2 is nuclearlocalized (Hall et al., 1998), is able to transactivate reporter gene expression, and can both homo-dimerize and heterodimerize with ZmGLK1 (Rossini et al., 2001). DNA-binding activity of GLK proteins has yet to be demonstrated, however, the putative DNA-binding domain is highly conserved with domains in other GARP proteins such as ARR1 and ARR2 (Riechmann et al., 2000). Notably, ARR1 and ARR2 have been shown to bind DNA (Sakai et al.,2000), thus it is likely that GLK proteins act as transcriptional regulators of chloroplast development.

The GLK proteins are members of the GARP superfamily of transcription factors, and phylogenetic analysis demonstrates that the maize, rice and Arabidopsis GLK gene pairs comprise a distinct group within the GARP superfamily. Further phylogenetic analysis suggests that the gene pairs arose through separate duplication events in the monocot and dicot lineages. As in rice, AtGLK1 and AtGLK2 are expressed in partially overlapping domains in photosynthetic tissue. GLK genes therefore regulate chloroplast development in diverse plant species.

Papers

Eshed, Y; Baum, SF; Perea, JV; Bowman, JL. Establishment of polarity in lateral organs of plants. Curr. Biol. 2001. 11(16):1251-60 PMID: 11525739

Rossini, L; Cribb, L; Martin, DJ; Langdale, JA. The maize golden2 gene defines a novel class of transcriptional regulators in plants. Plant Cell 2001. 13(5):1231-44 PMID: 11340194



Searched with 10 DNA-binding domain sequences n.b these are MYB-like

Searched with the MYB-like domain.

The consensus is different from R2R3MYBS and MYB-like proteins.

PL_WTP_LH_RFV_AV__LGG___(intron)ATPK_____M___GLT____SHLQ_YR___

n.b SHLQ not SHAQ (MYB-related).

Number of contigs: 70

Number of singlets: 14

1 aa – 26 aa – 20

27 aa – end – 20

Number of full – 44

Total minimum number - 64




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
GARP-G2_1 [alias=GARP-G2-like_1] [comment=26-almost end] ET042712
GARP-G2_2 [alias=GARP-G2-like_2] [comment=1-26] ET051540
ET051495
GARP-G2_3 [alias=GARP-G2-like_3] [comment=26 to end. Divergent] ET043062
ET042357
GARP-G2_4 [alias=GARP-G2-like_4] [comment=26 to end] ET046624
ET048349
GARP-G2_5 [alias=GARP-G2-like_5] [comment=26 - end] ET051475
ET048994
GARP-G2_6 [alias=GARP-G2-like_6] [comment=first 26] ET041968
ET046382
GARP-G2_7 [alias=GARP-G2-like_7] [comment=26-end] ET043762
ET050629
GARP-G2_8 [alias=GARP-G2-like_8] [comment=full] ET049639
ET048724
GARP-G2_9 [alias=GARP-G2-like_9] [comment=full] ET046135
ET049881
GARP-G2_10 [alias=GARP-G2-like_10] [comment=28-end] ET042232
ET049142
GARP-G2_11 [alias=GARP-G2-like_11] [comment=26-end] ET047662
ET047661
GARP-G2_12 [alias=GARP-G2-like_12] [comment=first 26] ET051166
ET051167
GARP-G2_13 [alias=GARP-G2-like_13] [comment=full] ET043368
ET050249
GARP-G2_14 [alias=GARP-G2-like_14] [comment=26 to end] ET042711
ET042368
GARP-G2_15 [alias=GARP-G2-like_15] [comment=full] ET050425
ET042219
GARP-G2_16 [alias=GARP-G2-like_16] [comment=1-26 divergent] ET043681
ET043670
GARP-G2_17 [alias=GARP-G2-like_17] [comment=full] ET047995
ET050138
GARP-G2_18 [alias=GARP-G2-like_18] [comment=1-26] ET042524
ET043283
GARP-G2_19 [alias=GARP-G2-like_19] [comment=1-26] ET051839
ET048632
GARP-G2_20 [alias=GARP-G2-like_20] [comment=1-26] ET044492
ET047966
GARP-G2_21 [alias=GARP-G2-like_21] [comment=27 - end] ET043449
ET043450
ET043136
GARP-G2_22 [alias=GARP-G2-like_22] [comment=27 - end] ET051273
ET046210
ET046330
GARP-G2_23 [alias=GARP-G2-like_23] [comment=full] ET044125
ET042671
ET042670
GARP-G2_24 [alias=GARP-G2-like_24] [comment=1-26] ET042912
ET042832
ET049887
GARP-G2_25 [alias=GARP-G2-like_25] [comment=full except missing last 10 amino acids] ET048513
ET047919
ET046442
GARP-G2_26 [alias=GARP-G2-like_26] [comment=full] ET042997
ET042431
ET044318
GARP-G2_27 [alias=GARP-G2-like_27] [comment=27-end] ET044254
ET048705
ET042396
GARP-G2_28 [alias=GARP-G2-like_28] [comment=1-26] ET050035
ET043502
ET050878
GARP-G2_29 [alias=GARP-G2-like_29] [comment=full but divergent] ET045106
ET050538
ET046774
GARP-G2_30 [alias=GARP-G2-like_30] [comment=1-26] ET051820
ET047090
ET051427
GARP-G2_31 [alias=GARP-G2-like_31] [comment=27-end] ET047867
ET049520
ET049249
GARP-G2_32 [alias=GARP-G2-like_32] [comment=full] ET044035
ET042141
ET051288
GARP-G2_33 [alias=GARP-G2-like_33] [comment=full] ET050104
ET047872
ET042740
GARP-G2_34 [alias=GARP-G2-like_34] [comment=full] ET048149
ET047211
ET047210
GARP-G2_35 [alias=GARP-G2-like_35] [comment=full] ET047625
ET048987
ET048986
GARP-G2_36 [alias=GARP-G2-like_36] [comment=full] ET050671
ET047160
ET045960
GARP-G2_37 [alias=GARP-G2-like_37] [comment=27-end divergent] ET049471
ET049955
ET047173
GARP-G2_38 [alias=GARP-G2-like_38] [comment=full] ET049823
ET047809
ET051431
GARP-G2_39 [alias=GARP-G2-like_39] [comment=27-end divergent] ET047053
ET048406
ET047052
GARP-G2_40 [alias=GARP-G2-like_40] [comment=full divergent] ET051060
ET051059
ET044780
GARP-G2_41 [alias=GARP-G2-like_41] [comment=full] ET044664
ET044121
ET044665
GARP-G2_42 [alias=GARP-G2-like_42] [comment=full] ET047087
ET046793
ET045014
GARP-G2_43 [alias=GARP-G2-like_43] [comment=full] ET048034
ET051314
ET042836
ET045319
GARP-G2_44 [alias=GARP-G2-like_44] [comment=full] ET044835
ET047968
ET049203
ET048792
GARP-G2_45 [alias=GARP-G2-like_45] [comment=full divergent] ET046568
ET047193
ET051867
ET045291
GARP-G2_46 [alias=GARP-G2-like_46] [comment=1-26] ET049139
ET047265
ET047266
ET046177
GARP-G2_47 [alias=GARP-G2-like_47] [comment=full] ET047168
ET049641
ET047167
ET049642
GARP-G2_48 [alias=GARP-G2-like_48] [comment=full very similar to NbPCL1] ET045570
ET045551
ET045542
ET051168
GARP-G2_49 [alias=GARP-G2-like_49] [comment=full divergent] ET044478
ET043659
ET042289
ET042288
GARP-G2_50 [alias=GARP-G2-like_50] [comment=full] ET044066
ET046111
ET046496
ET046029
GARP-G2_51 [alias=GARP-G2-like_51] [comment=full] ET043510
ET048171
ET047223
ET042048
GARP-G2_52 [alias=GARP-G2-like_52] [comment=full divergent] ET042191
ET047659
ET047658
ET047660
GARP-G2_53 [alias=GARP-G2-like_53] [comment=full] ET047852
ET047971
ET048567
ET042026
GARP-G2_54 [alias=GARP-G2-like_54] [comment=full] ET051474
ET051530
ET048508
ET044182
GARP-G2_55 [alias=GARP-G2-like_55] [comment=full] ET042399
ET050291
ET051432
ET043572
GARP-G2_56 [alias=GARP-G2-like_56] [comment=1-26] ET048195
ET043153
ET042837
ET042838
GARP-G2_57 [alias=GARP-G2-like_57] [comment=27-end divergent] ET047967
ET044180
ET049375
ET051707
GARP-G2_58 [alias=GARP-G2-like_58] [comment=full] ET049499
ET051827
ET047159
ET049500
GARP-G2_59 [alias=GARP-G2-like_59] [comment=full] ET051565
ET044292
ET051564
ET043475
GARP-G2_60 [alias=GARP-G2-like_60] [comment=full] ET047703
ET047704
ET047705
ET051588
ET045919
GARP-G2_61 [alias=GARP-G2-like_61] [comment=full] ET045007
ET047882
ET051463
ET050871
ET043997
GARP-G2_62 [alias=GARP-G2-like_62] [comment=full] ET044231
ET045003
ET044230
ET049252
ET046176
GARP-G2_63 [alias=GARP-G2-like_63] [comment=full] ET043451
ET043452
ET051428
ET050556
ET050557
GARP-G2_64 [alias=GARP-G2-like_64] [comment=full] ET046121
ET047632
ET047633
ET051280
ET044623
GARP-G2_65 [alias=GARP-G2-like_65] [comment=full but divergent] ET046938
ET048702
ET046386
ET042214
ET043164
GARP-G2_66 [alias=GARP-G2-like_66] [comment=full] ET047766
ET049705
ET051538
ET047767
ET043140
ET049706
GARP-G2_67 [alias=GARP-G2-like_67] [comment=1-26] ET043501
ET043500
ET045572
ET048030
ET043499
ET042186
ET042819
GARP-G2_68 [alias=GARP-G2-like_68] [comment=full] ET050074
ET045183
ET041693
ET041806
ET041805
ET044862
ET041692
GARP-G2_69 [alias=GARP-G2-like_69] [comment=full] ET044233
ET050451
ET044232
ET046600
ET043824
ET043074
ET050404
ET047721
GARP-G2_70 [alias=GARP-G2-like_70] [comment=full] ET044513
ET049405
ET042120
ET044514
ET046647
ET045182
ET043414
ET048913
ET048912
ET049404
GARP-G2_71 [alias=GARP-G2-like_71] [comment=1-26] ET046448
GARP-G2_72 [alias=GARP-G2-like_72] [comment=27-end] ET043406
GARP-G2_73 [alias=GARP-G2-like_73] [comment=1-26] ET044537
GARP-G2_74 [alias=GARP-G2-like_74] [comment=1-24 divergent] ET051278
GARP-G2_75 [alias=GARP-G2-like_75] [comment=1-26] ET048811
GARP-G2_76 [alias=GARP-G2-like_76] [comment=missing first 10 amino acids] ET043579
GARP-G2_77 [alias=GARP-G2-like_77] [comment=1-26] ET044489
GARP-G2_78 [alias=GARP-G2-like_78] [comment=27-end] ET050539
GARP-G2_79 [alias=GARP-G2-like_79] [comment=1-26] ET043405
GARP-G2_80 [alias=GARP-G2-like_80] [comment=1-26] ET051446
GARP-G2_81 [alias=GARP-G2-like_81] [comment=full] ET043315
GARP-G2_82 [alias=GARP-G2-like_82] [comment=27-end] ET051666
GARP-G2_83 [alias=GARP-G2-like_83] [comment=27-end] ET044749
GARP-G2_84 [alias=GARP-G2-like_84] [comment=27-end] ET044957


Tobacco published genes related to transcription factor family GARP-G2
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu