GeBP Genomic sequences

Minimum number of tobacco GeBP genes: 15

Count of tobacco GeBP sequences: 16

Pfam accession: DUF573

SHOULD possess DUF573 domain

Trichomes of Arabidopsis are single-celled epidermal hair that are a useful model for studying plant cell fate determination. Trichome initiation requires the activity of the GLABROUS1 (GL1) gene whose expression in epidermal and trichome cells is dependent on the presence of a 3'-cis-regulatory element. Using a one-hybrid screen, we have isolated a cDNA, which encodes for a protein, GL1 enhancer binding protein (GeBP), that binds this regulatory element in yeast and in vitro. GeBP and its three homologues in Arabidopsis share two regions: a central region with no known motifs and a C-terminal region with a putative leucine-zipper motif. We show that both regions are necessary for trans-activation in yeast. A translational fusion with the Yellow Fluorescent Protein (YFP) indicates that GeBP is a nuclear protein whose localization is restricted to, on average, 3-5 subnuclear foci that might correspond to nucleoli. Transcriptional fusion with the GUS reporter indicates that GeBP is mainly expressed in vegetative meristematic tissues and in very young leaf primordia. We looked at GeBP expression in plants mutated in or misexpressing KNAT1, a KNOX gene, expressed in the shoot apical meristem and downregulated in leaf founder cells, and found that GeBP transcript level is regulated by KNAT1 suggesting that KNAT1 is a transcriptional activator of GeBP. This regulation suggests that GeBP is acting as a repressor of leaf cell fate.

Papers

Curaba, J; Herzog, M; Vachon, G. GeBP, the first member of a new gene family in Arabidopsis, encodes a nuclear protein with DNA-binding activity and is regulated by KNAT1. Plant J. 2003. 33(2):305-17 PMID: 12535344



Number of contigs: 15

Number of singlets: 4

Number of N terminal – 1

Number of C terminal – 1

Number of full – 14

Total minimum number – 15




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
GeBP_1 [comment=full] ET049055
ET049902
GeBP_2 [comment=full] ET046913
ET050272
GeBP_3 [comment=full] ET046122
ET046123
GeBP_4 [comment=full] ET050855
ET046134
GeBP_5 [comment=C terminal] ET042465
ET042466
GeBP_6 [comment=full] ET042792
ET046756
ET042374
GeBP_7 [comment=full] ET043748
ET046362
ET049011
ET049842
GeBP_8 [comment=full] ET049282
ET051642
ET044859
ET049281
ET044110
GeBP_9 [comment=full] ET050709
ET046783
ET048905
ET051764
ET044594
GeBP_10 [comment=full] ET042877
ET046841
ET049678
ET042876
ET046840
GeBP_11 [comment=full] ET050099
ET043979
ET043511
ET050098
ET043154
GeBP_12 [comment=full] ET047409
ET047627
ET047628
ET042937
ET051797
ET044014
ET045625
GeBP_13 [comment=full] ET047484
ET043476
ET045596
ET045605
ET045379
ET042728
ET048079
ET047542
ET042976
ET045783
GeBP_14 [comment=full] ET050540
ET047984
ET049941
ET045972
ET049655
ET050681
ET047306
ET043346
ET044087
ET047375
GeBP_15 [comment=N terminal] ET046550
GeBP_16 [comment=full] ET042231


Tobacco published genes related to transcription factor family GeBP
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu