HRT Genomic sequences

Minimum number of tobacco HRT genes: 2

Count of tobacco HRT sequences: 4

Pfam accession: HRT

SHOULD possess HRT domain

A barley gene encoding a novel DNA-binding protein (HRT) was identified by southwestern screening with baits containing a gibberellin phytohormone response element from an alpha-amylase promoter. The HRT gene contains two introns, the larger of which (5722 base pairs (bp)) contains a 3094-bp LINE-like element with homology to maize Colonist1. In vitro mutagenesis and zinc- and DNA-binding assays demonstrate that HRT contains three unusual zinc fingers with a CX8-9CX10CX2H consensus sequence. HRT is targeted to nuclei, and homologues are expressed in other plants. In vivo, functional tests in plant cells indicate that full-length HRT can repress expression from certain promoters including the Amy1/6-4 and Amy2/32 alpha-amylase promoters. In contrast, truncated forms of HRT containing DNA-binding domains can activate, or derepress, transcription from these promoters. Northern hybridizations indicate that HRT mRNA accumulates to low levels in various tissues. Roles for HRT in mediating developmental and phytohormone-responsive gene expression are discussed.

Papers

Raventós, D; Skriver, K; Schlein, M; Karnahl, K; Rogers, SW; Rogers, JC; Mundy, J. HRT, a novel zinc finger, transcriptional repressor from barley. J. Biol. Chem. 1998. 273(36):23313-20 PMID: 9722564



Number of contigs: 4

Number of singlets: 0

Number of N terminal – 2

Number of C terminal – 2

Total minimum number – 2




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
HRT_1 3 prime end ET042996
ET047007
ET048703
HRT_2 5 prime end ET046277
ET050511
ET050512
ET050340
ET046278
HRT_3 5 prime end ET047174
ET045492
ET050559
ET045418
ET049211
HRT_4 3 prime end ET050341
ET045564
ET045419
ET044113
ET051610
ET047956
ET046349


Tobacco published genes related to transcription factor family HRT
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu