TOBFAC: The database of tobacco transcription factors
Family: HRT
Minimum number of tobacco HRT genes: 2
Count of tobacco HRT sequences: 4
Pfam accession: HRT
SHOULD possess HRT domain
A barley gene encoding a novel DNA-binding protein (HRT) was identified by southwestern screening with baits containing a gibberellin phytohormone response element from an alpha-amylase promoter. The HRT gene contains two introns, the larger of which (5722 base pairs (bp)) contains a 3094-bp LINE-like element with homology to maize Colonist1. In vitro mutagenesis and zinc- and DNA-binding assays demonstrate that HRT contains three unusual zinc fingers with a CX8-9CX10CX2H consensus sequence. HRT is targeted to nuclei, and homologues are expressed in other plants. In vivo, functional tests in plant cells indicate that full-length HRT can repress expression from certain promoters including the Amy1/6-4 and Amy2/32 alpha-amylase promoters. In contrast, truncated forms of HRT containing DNA-binding domains can activate, or derepress, transcription from these promoters. Northern hybridizations indicate that HRT mRNA accumulates to low levels in various tissues. Roles for HRT in mediating developmental and phytohormone-responsive gene expression are discussed.
Raventós, D; Skriver, K; Schlein, M; Karnahl, K; Rogers, SW; Rogers, JC; Mundy, J. HRT, a novel zinc finger, transcriptional repressor from barley. J. Biol. Chem. 1998. 273(36):23313-20 PMID: 9722564
Number of contigs: 4
Number of singlets: 0
Number of N terminal – 2
Number of C terminal – 2
Total minimum number – 2
|
Locus |
Description |
NCBI |
| HRT_1 | 3 prime end |
ET042996 ET047007 ET048703 |
| HRT_2 | 5 prime end |
ET046277 ET050511 ET050512 ET050340 ET046278 |
| HRT_3 | 5 prime end |
ET047174 ET045492 ET050559 ET045418 ET049211 |
| HRT_4 | 3 prime end |
ET050341 ET045564 ET045419 ET044113 ET051610 ET047956 ET046349 |
| Family | Genbank ID | Name |
Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko