HSF Genomic sequences

Minimum number of tobacco HSF genes: 34

Count of tobacco HSF sequences: 36

Pfam accession: HSF_DNA-bind

SHOULD possess HSF_DNA-bind domain

Organisms respond to elevated temperatures and to chemical and physiological stresses by an increase in the synthesis of heat shock proteins. The regulation of heat shock gene expression in eukaryotes is mediated by the conserved heat shock transcription factor (HSF). HSF is present in a latent state under normal conditions; it is activated upon heat stress by induction of trimerization and high-affinity binding to DNA and by exposure of domains for transcriptional activity. Analysis of HSF cDNA clones from many species has defined structural and regulatory regions responsible for the inducible activities. The heat stress signal is thought to be transduced to HSF by changes in the physical environment, in the activity of HSF-modifying enzymes, or by changes in the intracellular level of heat shock proteins.

Heat shock factor (HSF) is a transcriptional activator of heat shock genes it binds specifically to heat shock promoter elements, which are palindromic sequences rich with repetitive purine and pyrimidine motifs. Under normal conditions, HSF is a homo-trimeric cytoplasmic protein, but heat shock activation results in relocalisation to the nucleus. Each HSF monomer contains one C-terminal and three N-terminal leucine zipper repeats. Point mutations in these regions result in disruption of cellular localisation, rendering the protein constitutively nuclear. Two sequences flanking the N-terminal zippers fit the consensus of a bi- partite nuclear localisation signal (NLS). Interaction between the N- and C-terminal zippers may result in a structure that masks the NLS sequences: following activation of HSF, these may then be unmasked, resulting in relocalisation of the protein to the nucleus. The DNA-binding component of HSF lies to the N-terminus of the first NLS region, and is referred to as the HSF domain.

Papers

Baniwal, SK; Bharti, K; Chan, KY; Fauth, M; Ganguli, A; Kotak, S; Mishra, SK; Nover, L; Port, M; Scharf, KD; Tripp, J; Weber, C; Zielinski, D; von Koskull-Döring, P. Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors. J. Biosci. 2004 29(4):471-87 PMID: 15625403

Fujita, A; Kikuchi, Y; Kuhara, S; Misumi, Y; Matsumoto, S; Kobayashi, H. Domains of the SFL1 protein of yeasts are homologous to Myc oncoproteins or yeast heat-shock transcription factor. Gene 1989. 85(2):321-8 PMID: 2697640

Nover, L; Scharf, KD; Gagliardi, D; Vergne, P; Czarnecka-Verner, E; Gurley, WB. The Hsf world: classification and properties of plant heat stress transcription factors. Cell Stress Chaperones 1996. 1(4):215-23 PMID: 9222607

Wu, C. Heat shock transcription factors: structure and regulation. Annu. Rev. Cell Dev. Biol. 1995. 11:441-69 PMID: 8689565



Number of contigs: 32

Number of singlets: 4

Number of N terminal – 1

Number of C terminal – 2

Number of middle bits – 1

Number of full – 32

Total minimum number – 34




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
HSF_1 [comment=C terminal] ET048585
HSF_2 [comment=full (NtHSF1)] ET049368
ET048841
ET044881
HSF_3 [comment=full] ET043755
ET051232
ET046256
HSF_4 [comment=full] ET043875
ET051663
ET048529
HSF_5 [comment=full] ET042104
ET042718
ET043675
HSF_6 [comment=full] ET049393
ET048293
ET046763
HSF_7 [comment=full] ET051225
ET051224
ET042294
HSF_8 [comment=full] ET051386
ET047131
ET048268
HSF_9 [comment=full] ET047296
ET048700
ET051573
HSF_10 [comment=full] ET042389
ET042388
ET044266
HSF_11 [comment=full] ET047560
ET051349
ET049144
HSF_12 [comment=full] ET041788
ET049882
ET051163
HSF_13 [comment=full ] ET042510
ET042509
ET046908
HSF_14 [comment=full] ET047096
ET048462
ET049269
ET044659
HSF_15 [comment=full] ET046940
ET045169
ET044554
ET045249
HSF_16 [comment=full] ET045853
ET048090
ET047621
ET046013
HSF_17 [comment=full] ET042805
ET048451
ET046708
ET042804
HSF_18 [comment=full] ET042928
ET042929
ET044538
ET044689
HSF_19 [comment=full] ET050934
ET049398
ET042355
ET043583
HSF_20 [comment=full] ET044675
ET046988
ET049461
ET049827
HSF_21 [comment=full] ET042378
ET041673
ET050639
ET050830
HSF_22 [comment=full] ET050952
ET049532
ET051848
ET044925
ET050951
HSF_23 [comment=full] ET042428
ET051757
ET050058
ET050672
ET048675
HSF_24 [comment=full] ET047277
ET049762
ET049446
ET042236
ET042235
HSF_25 [comment=full] ET051005
ET050010
ET048121
ET045494
ET045857
ET050790
HSF_26 [comment=full] ET046804
ET049764
ET049606
ET049911
ET043523
ET046803
HSF_27 [comment=full] ET043994
ET046687
ET047425
ET051858
ET046688
ET050877
HSF_28 [comment=full] ET049280
ET042861
ET044529
ET049423
ET050304
ET050319
ET045305
HSF_29 [comment=full] ET049268
ET050430
ET049509
ET050781
ET045111
ET048733
ET050780
HSF_30 [comment=full] ET049131
ET045153
ET045155
ET048617
ET045728
ET048776
ET042015
HSF_31 [comment=full] ET042611
ET046352
ET045090
ET046353
ET047299
ET042612
ET051895
ET045089
HSF_32 [comment=full] ET048989
ET048990
ET041711
ET041710
ET051286
ET046141
ET051133
ET051134
ET045627
ET051135
HSF_33 [comment=full] ET051126
HSF_34 [comment=C terminal] ET042531
HSF_35 [comment=middle bit] ET047097
HSF_36 [comment=N terminal] ET042154


Tobacco published genes related to transcription factor family HSF
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu