TOBFAC: The database of tobacco transcription factors
Family: MADS
Minimum number of tobacco MADS Box genes: 119
Count of tobacco MADS Box sequences: 122
Pfam accession: SRF-TF
SHOULD possess SRF-TF domain and COULD possess K-box domain
Members of the MADS box family are known to be predominately involved in developmental processes and are found in animal as well as plant species. In plants, the gene family has greatly expanded and this appears to have been instrumental in shaping the evolution of the true flower around 120-150 million years ago . As with other families of transcription factors, these genes share a common DNA binding domain, in this case the MADS box domain. MADS box factors bind to the CArG box (CC(A/T)6GG) that shows dyad symmetry . MADS box proteins form multimeric protein complexes that interact with promoter sequences on their target genes. Many of these interactions are between MADS box genes but interactions also occur with other types of protein . The first two MADS box genes to be discovered in plants were DEFICIENS (DEF) from Antirrhinum majus and AGAMOUS (AG) from Arabidopsis and work on MADS box genes and floral organ formation has led to the ABCDE model that explains how transcription factors acting alone or in combination can lead to the formation of floral organs. All of these transcription factors except APETELA2 are MADS box genes.
Phylogenetic analysis of MADS box genes from fungi, animals and plants, suggests that there are two main lineages, type I and type II, caused by a gene duplication that occurred before the divergence of plants and animals . The complete Arabidopsis gene family demonstrates that MADS box genes can be further divided into five subgroups (MIKC, M, M, M, and M) based on phylogenetic relationships of the MADS box domain . Most known MADS box mutants are found in the MIKC group which together with M make up the type II genes.
Parenicova L, de Folter S, Kieffer M, Horner DS, Favalli C, Busscher J, Cook HE, Ingram RM, Kater MM, Davies B, Angenent GC, Colombo L. Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: new openings to the MADS world. Plant Cell. 2003 Jul;15(7):1538-51. PMID: 12837945
Nam J, dePamphilis CW, Ma H, Nei M. Antiquity and evolution of the MADS-box gene family controlling flower development in plants. Mol Biol Evol. 2003 Sep;20(9):1435-47. Epub 2003 May 30. PMID: 12777513
Ng, M; Yanofsky, MF. Function and evolution of the plant MADS-box gene family. Nat. Rev. Genet. 2001 2(3):186-95 PMID: 11256070
Riechmann, JL; Meyerowitz, EM. MADS domain proteins in plant development. Biol. Chem. 1997. 378(10):1079-101 PMID: 9372178
Number of contigs: 115
Number of singlets: 7
Number of N terminal – 0
Number of C terminal – 9
Number of full – 107
Number of published (present in GSS but lacking MADS domain) – 3
Total minimum number – 119
| Family | Genbank ID | Name |
| MADS | DQ534202 | FUL |
| MADS | X96428 | MADS-box |
| MADS | X76188 | tobmads1 |
| MADS | X67959 | NTGLOBOSA |
| MADS | AY183721 | Mads1;11 |
| MADS | AF385746 | MADS11 |
| MADS | AF068723 | NtMADS4 |
| MADS | AF068724 | NtMADS5 |
| MADS | AF009127 | NAP1-2 |
| MADS | AF009126 | NAP1-1 |
| MADS | TOBNAG1A | NAG1 |
Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko
