PLATZ Genomic sequences

Minimum number of tobacco PLATZ genes: 15

Count of tobacco PLATZ sequences: 21

Pfam accession: DUF597

SHOULD possess DUF597 domain

Complementary DNA encoding a DNA-binding protein, designated PLATZ1 (plant AT-rich sequence- and zinc-binding protein 1), was isolated from peas. The amino acid sequence of the protein is similar to those of other uncharacterized proteins predicted from the genome sequences of higher plants. However, no paralogous sequences have been found outside the plant kingdom. Multiple alignments among these paralogous proteins show that several cysteine and histidine residues are invariant, suggesting that these proteins are a novel class of zinc-dependent DNA-binding proteins with two distantly located regions, C-x(2)-H-x(11)-C-x(2)-C-x((4-5))-C-x(2)-C-x((3-7))-H-x(2)-H and C-x(2)-C-x((10-11))-C-x(3)-C. In an electrophoretic mobility shift assay, the zinc chelator 1,10-o-phenanthroline inhibited DNA binding, and two distant zinc-binding regions were required for DNA binding. A protein blot with (65)ZnCl(2) showed that both regions are required for zinc-binding activity. The PLATZ1 protein non-specifically binds to A/T-rich sequences, including the upstream region of the pea GTPase pra2 and plastocyanin petE genes. Expression of the PLATZ1 repressed those of the reporter constructs containing the coding sequence of luciferase gene driven by the cauliflower mosaic virus (CaMV) 35S90 promoter fused to the tandem repeat of the A/T-rich sequences. These results indicate that PLATZ1 is a novel class of plant-specific zinc-dependent DNA-binding protein responsible for A/T-rich sequence-mediated transcriptional repression.

Papers

Nagano, Y; Furuhashi, H; Inaba, T; Sasaki, Y. A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. Nucleic Acids Res. 2001. 29(20):4097-105 PMID: 11600698



Number of contigs: 15

Number of singlets: 6

Number of N terminal – 4

Number of C terminal – 6

Number of full – 9

Number of genes with middle bits – 2

Total minimum number – 15




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
PLATZ_1 [comment=full] ET046504
PLATZ_2 [comment=N terminal] ET051418
ET049817
PLATZ_3 [comment=N terminal] ET046559
ET049592
PLATZ_4 [comment=C terminal] ET048211
ET043229
PLATZ_5 [comment=middle bit] ET050171
ET043330
PLATZ_6 [comment=C terminal] ET044242
ET044243
PLATZ_7 [comment=N terminal] ET048375
ET042710
PLATZ_8 [comment=full] ET044865
ET045278
ET045277
PLATZ_9 [comment=full] ET047722
ET042575
ET042574
ET048891
PLATZ_10 [comment=full] ET045488
ET042644
ET050628
ET045487
PLATZ_11 [comment=full] ET043529
ET043656
ET043655
ET043835
PLATZ_12 [comment=full] ET043111
ET047924
ET043947
ET049069
PLATZ_13 [comment=full] ET051125
ET050193
ET051534
ET045214
ET051483
ET051482
PLATZ_14 [comment=full] ET051181
ET044809
ET041942
ET051182
ET048774
ET041943
ET051098
PLATZ_15 [comment=full] ET046670
ET049020
ET046669
ET044021
ET049021
ET049745
ET051572
ET042037
PLATZ_16 [comment=C terminal] ET045884
PLATZ_17 [comment=C terminal] ET049593
PLATZ_18 [comment=C terminal] ET047630
PLATZ_19 [comment=C terminal] ET043105
PLATZ_20 [comment=middle bit] ET049458
PLATZ_21 [comment=N terminal] ET043653


Tobacco published genes related to transcription factor family PLATZ
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu