SRS Genomic sequences

Minimum number of tobacco SRS genes: 12

Count of tobacco SRS sequences: 12

Pfam accession: DUF702

SHOULD possess DUF702 domain

SHI RELATED SEQUENCE(SRS), which encode proteins with a single zinc finger motif. The genes are members of a small gene family of putative transcription factors in which the SHORT INTERNODES (SHI) gene is found.
The predicted amino acid sequence of SHI has acidic and glutamine-rich stretches and shows sequence similarity over a putative zinc finger region to three presumptive Arabidopsis proteins. This suggests that SHI may act as a negative regulator of GA responses through transcriptional control.
The predicted SHI protein consists of 331 amino acids. A region containing six cysteine residues (amino acids 120 to 147) in a C-X2-C-X7-C-X4-C-X2-C-X7-C arrangement (where X is a variable amino acid) is suggested to make up a zinc finger domain similar to the Zn2Cys6 cluster found in the DNA binding region of the yeast transcriptional activator GAL4 (Kraulis et al. 1992). Two regions rich in glutamine residues were found adjacent to the zinc finger domain, as well as a short stretch of acidic residues in the C-terminal third of the SHI protein. These features have been found in transcription factor proteins and have been demonstrated to be important for their function as transcriptional activators (Mitchell and Tjian 1989). Furthermore, two putative nuclear localization signals (NLSs) were found in the SHI sequence. These are two basic stretches (amino acids 160 to 165 and 183 to 188) that conform to the consensus of the simian virus 40¨Clike "typical NLS" defined as four arginines and lysines within a region of six amino acids (Boulikas 1994 ; LaCasse and Lefebvre 1995).

Papers

Fridborg, I; Kuusk, S; Robertson, M; Sundberg, E. The Arabidopsis protein SHI represses gibberellin responses in Arabidopsis and barley. Plant Physiol. 2001. 127(3):937-48 PMID: 11706176

Fridborg, I; Kuusk, S; Moritz, T; Sundberg, E. The Arabidopsis dwarf mutant shi exhibits reduced gibberellin responses conferred by overexpression of a new putative zinc finger protein. Plant Cell 1999. 11(6):1019-32 PMID: 10368174



Number of contigs: 11

Number of singlets: 1

Total minimum number – 12




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
SRS_1 ET046746
SRS_2 ET047156
SRS_3 ET048402
ET050460
ET048507
SRS_4 ET047634
ET049194
ET044740
SRS_5 ET049840
ET042798
ET043507
ET042799
SRS_6 ET049920
ET046264
ET048552
ET047427
ET046263
SRS_7 ET047806
ET048464
ET045144
ET045671
ET047805
SRS_8 ET043422
ET042427
ET045931
ET046145
ET047485
ET046578
SRS_9 ET047523
ET045198
ET043886
ET043620
ET045010
ET050647
SRS_10 ET049726
ET050981
ET050982
ET041818
ET041817
ET041913
ET049868
SRS_11 ET043782
ET051127
ET051128
ET051129
ET051794
ET043495
ET043834
ET050132
ET045222
SRS_12 ET046236


Tobacco published genes related to transcription factor family SRS
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu