TAZ Genomic sequences

Minimum number of tobacco TAZ genes: 8

Count of tobacco TAZ sequences: 8

Pfam accession: zf-TAZ

SHOULD possess zf-TAZ domain but SHOULD NOT possess PHD domain

A novel CaM-binding protein was isolated through protein-protein interaction based screening of an Arabidopsis cDNA expression library using a 35S calmodulin (CaM) probe. There are four additional homologs in the Arabidopsis genome with similar structures: a BTB domain in the N-terminus and a Zf-TAZ domain in the C-terminus. Hence, they were designated as AtBT1-5 (Arabidopsis thaliana BTB and TAZ domain protein). CaM-binding experiments revealed that all five AtBTs are CaM-binding proteins, and their CaM-binding domains were mapped to the C-terminus. AtBT homologs are also present in rice, but are not present in human, animal, yeast or other organisms, suggesting that the BTB and TAZ domain proteins are plant-specific. The AtBT1-smGFP fusion protein expressed in tobacco BY-2 cells showed that AtBT1 targets the nucleus. Yeast two-hybrid screening using an AtBT1 fragment as bait identified two interacting proteins (AtBET10 and AtBET9) belonging to the family of fsh/Ring3 class transcription regulators. The BTB domain of the AtBTs is required for the interaction, and this protein-protein interaction was confirmed by GST pull-down. AtBET10 also interacts with AtBT2 and AtBT4, and exhibited a transcriptional activation function in yeast cells. AtBTs exhibit varying responses to different stress stimuli, but all five genes responded rapidly to H2O2 and salicylic acid (SA) treatments. These results suggest that AtBTs play a role in transcriptional regulation, and signal molecules such as Ca2+, H2O2, and SA affect transcriptional machinery by altering the expression and conformation of AtBTs which interact with transcriptional activators such as AtBET10.

The TAZ2 (CH3) domain of the transcriptional adapter protein CBP has been implicated in direct functional interactions with numerous cellular transcription factors and viral oncoproteins. The solution structure of the TAZ2 domain of murine CBP has been determined by nuclear magnetic resonance (NMR). The protein adopts a novel helical fold stabilized by three zinc ions, each of which is bound to one histidine and three cysteine ligands in HCCC-type motifs. Each zinc-binding site is formed from the carboxy terminus of an alpha-helix, a short loop, and the amino terminus of the next alpha-helix. A peptide derived from the N-terminal transactivation domain of p53 binds specifically to one face of the TAZ2 domain. The close similarities between the TAZ2 and TAZ1 (CH1 domain of CBP/p300) sequences suggest that both domains will adopt similar three-dimensional structures.

Papers

Du, L; Poovaiah, BW. A novel family of Ca2+/calmodulin-binding proteins involved in transcriptional regulation: interaction with fsh/Ring3 class transcription activators. Plant Mol. Biol. 2004. 54(4):549-69 PMID: 15316289



JOURNAL OF MOLECULAR BIOLOGY 20;303(2):243-53 2000

Number of contigs: 8

Number of singlets: 0

Number of N terminal – 0

Number of C terminal – 3

Number of full – 5

Total minimum number – 8




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
TAZ_1 ET048858
ET043456
ET043534
TAZ_2 ET051459
ET043198
ET043238
TAZ_3 ET050164
ET048497
ET042082
ET043412
TAZ_4 [comment=3 prime end only] ET046898
ET051790
ET045120
ET046424
TAZ_5 [comment=3 prime end only] ET048357
ET042181
ET049978
ET046675
TAZ_6 ET049835
ET050295
ET047874
ET049836
TAZ_7 ET050224
ET049650
ET044795
ET047042
ET050669
TAZ_8 [comment=3 prime end] ET046789
ET050290
ET050289
ET042069
ET047999
ET050576
ET048821


Tobacco published genes related to transcription factor family TAZ
Family Genbank ID Name
TAZ NTU01961 CAP
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu