TULP Genomic sequences

Minimum number of tobacco TLP (TULP; TUBBY) genes: 13

Count of tobacco TLP (TULP; TUBBY) sequences: 23

Pfam accession: Tub

SHOULD possess Tub domain

In mammals, TUBBY-like proteins play an important role in maintenance and function of neuronal cells during postdifferentiation and development. We have identified a TUBBY-like protein gene family with 11 members in Arabidopsis, named AtTLP1-11. Although seven of the AtTLP genes are located on chromosome I, no local tandem repeats or gene clusters are identified. Except for AtTLP4, reverse transcription-PCR analysis indicates that all these genes are expressed in various organs in 6-week-old Arabidopsis. AtTLP1, 2, 3, 6, 7, 9, 10, and 11 are expressed ubiquitously in all the organs tested, but the expression of AtTLP5 and 8 shows dramatic organ specificity. These 11 family members share 30% to 80% amino acid similarities across their conserved C-terminal tubby domains. Unlike the highly diverse N-terminal region of animal TUBBY-like proteins, all AtTLP members except AtTLP8 contain a conserved F-box domain (51-57 residues). The interaction between AtTLP9 and ASK1 (Arabidopsis Skp1-like 1) is confirmed via yeast (Saccharomyces cerevisiae) two-hybrid assays. Abscisic acid (ABA)-insensitive phenotypes are observed for two independent AtTLP9 mutant lines, whereas transgenic plants overexpressing AtTLP9 are hypersensitive to ABA. These results suggest that AtTLP9 may participate in the ABA signaling pathway.

Papers

Boggon, TJ; Shan, WS; Santagata, S; Myers, SC; Shapiro, L. Implication of tubby proteins as transcription factors by structure-based functional analysis. Science 1999. 286(5447):2119-25 PMID: 10591637

Lai, CP; Lee, CL; Chen, PH; Wu, SH; Yang, CC; Shaw, JF. Molecular analyses of the Arabidopsis TUBBY-like protein gene family. Plant Physiol. 2004. 134(4):1586-97 PMID: 15064372

Santagata, S; Boggon, TJ; Baird, CL; Gomez, CA; Zhao, J; Shan, WS; Myszka, DG; Shapiro, L. G-protein signaling through tubby proteins. Science 2001. 292(5524):2041-50 PMID: 11375483



Number of contigs: 13

Number of singlets: 12

Number of N terminal – 10

Number of C terminal – 10

Number of full – 3

Total minimum number – 13




Search sequences and info


Transcription factor sequences
Locus
 Description
 NCBI
TULP_1 [comment=C terminal] ET045326
TULP_2 [comment=N terminal] ET044746
ET042180
TULP_3 [comment=C terminal] ET050886
ET044603
TULP_4 [comment=N terminal] ET046764
ET046765
TULP_5 [comment=C terminal] ET051190
ET051191
TULP_6 [comment=full] ET044197
ET044198
TULP_8 [comment=N terminal] ET043551
ET046572
ET050180
ET046571
TULP_9 [comment=full] ET046800
ET049917
ET046799
ET049916
TULP_10 [comment=C terminal] ET051153
ET051254
ET051154
ET043550
TULP_11 [comment=C terminal] ET045064
ET046962
ET051189
ET051188
ET045065
TULP_12 [comment=full] ET043471
ET046839
ET043470
ET049710
ET046693
TULP_13 [comment=N terminal] ET047275
ET047274
ET041831
ET041832
ET041824
ET043323
TULP_14 [comment=N terminal] ET046441
TULP_15 [comment=C terminal] ET045641
TULP_16 [comment=C terminal] ET046479
TULP_17 [comment=N terminal] ET051593
TULP_18 [comment=C terminal] ET042992
TULP_19 [comment=C terminal] ET051546
TULP_20 [comment=N terminal] ET043158
TULP_21 [comment=C terminal] ET045373
TULP_22 [comment=N terminal] ET050847
TULP_23 [comment=N terminal] ET048726
TULP_24 [comment=N terminal] ET043157


Tobacco published genes related to transcription factor family TULP
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu