Trihelix Genomic sequences

Minimum number of tobacco Trihelix genes: 40

Count of tobacco Trihelix sequences: 42

Pfam accession: trihelix

SHOULD possess trihelix domain

The trihelix domain is characteristic of the trihelix family. The trihelix domain was extracted from known members of this family in Nicotiana tabacum, Arabidopsis thaliana and Oryza sativa.

GT-2 is a plant transcriptional activator that contains two separate, but similar, trihelix DNA-binding domains. GT-1 is similar to GT-2, although it contains only one of such domains. cDNAs that encode GT-2 were isolated from rice (OS-GT2) and Arabidopsis (AT-GT2). Evidence is presented for the existence of an Arabidopsis gene family that is structurally related to AT-GT2. Two members of this GT2-like family, AT-GTL1 and AT-GTL2, have been isolated and characterized. Their sequences suggest that they evolved by a recent gene duplication event. Both AT-GT2 and AT-GTL genes contain an intron in the amino-terminal trihelix motif, indicating that this DNA-binding domain resulted from exon shuffling. RNA gel blot analysis using AT-GTL1 as a probe revealed four transcripts in the aerial part of the plant. All mRNA levels were significantly higher in siliques, suggesting that this gene family may function in fruit and/or seed development. To date, DNA-binding proteins characterized by the trihelix motif have been described only in plants, and may therefore be involved in plant-specific processes. Our results show that in Arabidopsis thaliana, the trihelix motif is not restricted to the GT-1 and GT-2 DNA-binding proteins.

Papers

Nagano, Y. Several features of the GT-factor trihelix domain resemble those of the Myb DNA-binding domain. Plant Physiol. 2000. 124(2):491-4 PMID: 11027698

Nagano, Y; Inaba, T; Furuhashi, H; Sasaki, Y. Trihelix DNA-binding protein with specificities for two distinct cis-elements: both important for light down-regulated and dark-inducible gene expression in higher plants. J. Biol. Chem. 2001. 276(25):22238-43 PMID: 11301338

Smalle, J; Kurepa, J; Haegman, M; Gielen, J; Van Montagu, M; Van Der Straeten, D. The trihelix DNA-binding motif in higher plants is not restricted to the transcription factors GT-1 and GT-2. Proc. Natl. Acad. Sci. U.S.A. 1998. 95(6):3318-22 PMID: 9501260

Zhou, DX. Regulatory mechanism of plant gene transcription by GT-elements and GT-factors. Trends Plant Sci 1999. 4(6):210-214 PMID: 10366876



Number of contigs: 37

Number of singlets: 7

Number of N terminal – 4

Number of C terminal – 2

Number of full – 36

Total minimum number – 40






Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
Trihelix_1 [comment=full] ET050922
ET046313
Trihelix_2 [comment=full] ET049866
ET041678
Trihelix_3 [comment=full] ET045697
ET051638
Trihelix_4 [comment=full] ET044972
ET044509
Trihelix_5 [comment=full] ET050927
ET048943
Trihelix_6 [comment=full] ET046808
ET042754
Trihelix_7 [comment=N terminal] ET051305
ET048153
Trihelix_8 [comment=full] ET050021
ET050247
Trihelix_9 [comment=full] ET051283
ET050343
ET050301
Trihelix_10 [comment=full] ET042919
ET049626
ET045095
Trihelix_11 [comment=full] ET048549
ET048834
ET045690
Trihelix_12 [comment=full] ET050322
ET050276
ET048605
Trihelix_13 [comment=full] ET043829
ET049739
ET047795
Trihelix_14 [comment=full] ET051590
ET042261
ET046418
Trihelix_15 [comment=N terminal] ET047646
ET041690
ET041839
Trihelix_16 [comment=full] ET041969
ET050569
ET043665
ET045895
Trihelix_17 [comment=full] ET043948
ET045723
ET041724
ET041768
Trihelix_18 [comment=full] ET048214
ET048280
ET044161
ET051513
Trihelix_19 [comment=full] ET051883
ET050239
ET044426
ET042961
Trihelix_20 [comment=full] ET046136
ET043893
ET046961
ET048627
Trihelix_21 [comment=full] ET044264
ET043426
ET044301
ET049682
Trihelix_22 [comment=full] ET045737
ET041932
ET041933
ET046110
Trihelix_23 [comment=full] ET046332
ET046523
ET048717
ET043045
ET051319
Trihelix_24 [comment=N terminal] ET042885
ET041727
ET041992
ET042886
ET041993
Trihelix_25 [comment=full] ET041970
ET044755
ET050881
ET044020
ET049648
Trihelix_26 [comment=full] ET047166
ET042238
ET045585
ET051344
ET042106
Trihelix_27 [comment=full] ET043093
ET045516
ET043092
ET046112
ET041821
Trihelix_28 [comment=full] ET050820
ET047502
ET051647
ET043939
ET044991
Trihelix_29 [comment=full] ET044007
ET051742
ET046006
ET051242
ET042134
Trihelix_30 [comment=full] ET051023
ET047545
ET041996
ET051051
ET041997
Trihelix_31 [comment=full] ET047505
ET050096
ET051295
ET046015
ET046014
Trihelix_32 [comment=full] ET051186
ET042888
ET049627
ET045524
ET051630
ET042887
Trihelix_33 [comment=full (NtSIP1 - 6b interacting protein1)] ET045497
ET048955
ET045496
ET045977
ET045976
ET051777
ET044531
Trihelix_34 [comment=full] ET051599
ET050375
ET044063
ET044733
ET045122
ET045123
ET044064
ET048242
ET046298
Trihelix_35 [comment=full] ET047651
ET051497
ET041772
ET044931
ET047860
ET045844
ET041721
ET044849
ET050362
Trihelix_36 [comment=full] ET049672
ET048228
ET048246
ET049385
ET043612
ET041725
ET043800
ET041869
ET041870
ET043861
ET049091
ET050542
ET044712
ET047318
ET050921
ET045601
ET042434
Trihelix_37 [comment=full] ET051208
ET045983
ET041751
ET044527
ET045696
ET049086
ET044921
ET043277
ET046168
ET047368
ET051703
ET051209
ET041752
ET045984
ET046167
ET049085
ET043278
ET048048
ET042400
Trihelix_38 [comment=full] ET043521
Trihelix_39 [comment=N terminal] ET046952
Trihelix_40 [comment=C terminal] ET045224
Trihelix_41 [comment=full] ET048550
Trihelix_42 [comment=C terminal] ET044829


Tobacco published genes related to transcription factor family Trihelix
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu