ZF-HD Genomic sequences

Minimum number of tobacco ZF-HD genes: 38

Count of tobacco ZF-HD sequences: 39

Pfam accession: ZF-HD_dimer

SHOULD possess ZF-HD_dimer domain

This group of sequences described by a 54-residue domain found in the N-terminal region of plant proteins, the vast majority of which contain a ZF-HD class homeobox domain toward the C terminus. The region between the two domains typically is rich in low complexity sequence.

A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase (C4 phosphoenolpyruvate carboxylase) gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Papers

Windhövel, A; Hein, I; Dabrowa, R; Stockhaus, J. Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia. Plant Mol. Biol. 2001. 45(2):201-14 PMID: 11289511



Number of contigs: 39

Number of singlets: 0

Number of N terminal – 1

Number of C terminal – 4

Number of full – 34

Total minimum number – 38




Search sequences and info


Transcription factor sequences
Locus
Description
NCBI
ZF-HD_1 [comment=half of the domain (C terminal)] ET045247
ZF-HD_2 [comment=full] ET048620
ZF-HD_3 [comment=half of the domain (C terminal)] ET045729
ZF-HD_4 [comment=full] ET048619
ZF-HD_5 [comment=full] ET041782
ET041781
ZF-HD_6 [comment=full] ET046620
ET047069
ZF-HD_7 [comment=full] ET048738
ET043717
ZF-HD_8 [comment=half of the domain (N terminal)] ET042714
ET044043
ZF-HD_9 [comment=full] ET050251
ET047054
ZF-HD_10 [comment=half of the domain (C terminal)] ET047364
ET049939
ZF-HD_11 [comment=full] ET050250
ET047624
ZF-HD_12 [comment=full] ET045450
ET049416
ET051030
ZF-HD_13 [comment=full] ET043156
ET049526
ET044702
ZF-HD_14 [comment=full] ET045614
ET047300
ET050738
ZF-HD_15 [comment=full] ET043168
ET045706
ET045713
ZF-HD_16 [comment=full] ET051779
ET045406
ET046375
ZF-HD_17 [comment=full] ET046207
ET045197
ET046208
ZF-HD_18 [comment=full] ET051699
ET049219
ET041918
ZF-HD_19 [comment=full] ET044178
ET047648
ET048283
ZF-HD_20 [comment=half of the domain (C terminal)] ET047912
ET044274
ET044757
ET044375
ZF-HD_21 [comment=full] ET045099
ET043668
ET046196
ET048963
ZF-HD_22 [comment=full] ET049266
ET046118
ET046117
ET051117
ZF-HD_23 [comment=full] ET051806
ET044934
ET047342
ET047519
ZF-HD_24 [comment=full] ET051072
ET051071
ET045260
ET049498
ZF-HD_25 [comment=full] ET044600
ET044391
ET051388
ET050242
ZF-HD_26 [comment=full] ET046081
ET047292
ET047113
ET050313
ET043929
ZF-HD_27 [comment=full] ET048782
ET048847
ET045937
ET043443
ET050988
ZF-HD_28 [comment=full] ET045036
ET043162
ET051643
ET043912
ET049063
ZF-HD_29 [comment=full] ET042423
ET042422
ET043480
ET050038
ET049703
ET048950
ZF-HD_30 [comment=full] ET046639
ET044980
ET043768
ET045628
ET046640
ET045015
ZF-HD_31 [comment=full ] ET045927
ET042840
ET041775
ET041774
ET045624
ET042839
ZF-HD_32 [comment=full] ET049430
ET043690
ET049510
ET049511
ET045162
ET047332
ET045161
ZF-HD_33 [comment=full] ET043163
ET051719
ET043927
ET043646
ET041764
ET050862
ET050861
ZF-HD_34 [comment=full] ET043147
ET049570
ET048305
ET044981
ET045995
ET043419
ET049390
ZF-HD_35 [comment=full] ET047556
ET051718
ET048013
ET051302
ET042147
ET050069
ET050079
ZF-HD_36 [comment=full] ET044229
ET048314
ET045019
ET045018
ET043639
ET050402
ET047383
ET047463
ZF-HD_37 [comment=full] ET051042
ET050987
ET050986
ET045046
ET045693
ET051041
ET044313
ET045045
ZF-HD_38 [comment=full] ET045898
ET045870
ET046528
ET046186
ET043839
ET043554
ET049023
ET043199
ET046251
ET042965
ET044046
ZF-HD_39 [comment=full] ET050150
ET050789
ET050788
ET044051
ET046446
ET046447
ET045753
ET051021
ET046934
ET044052
ET045261
ET050149


Tobacco published genes related to transcription factor family ZF-HD
Family Genbank ID Name
Authors of this site:

Paul J Rushton
Marta T. Bokowiec
Xianfeng (Jeff) Chen
Thomas (Tom) W Laudeman
Jennifer F. Brannock
Michael P. Timko

Contact:

pr8y@virginia.edu